PXD010035-1

PXD010035 is an original dataset announced via ProteomeXchange.

Title	Glycoproteome of Human lung cancer cell by LC-MSMS
Description	We demonstrate the utility of lectin-magnetic nanoprobe coupled with Orbitrap HCD-CID-MS/MS for complementary glycotope-specific enrichment and site-specific glycosylation analysis of the glycoproteome. Using non-small cell lung cancer (NSCLC) cells as model, this approach allowed us to compare the glycosylation profiles of the drug resistant cell, PC9, and its resistant derivative, PC9-IR. The complementary enrichment allowed broader targeting of different glycoforms; using Byonic software, confident identification of a total of 2767 and 2290 non-redundant intact glycopeptides was achieved for PC9-IR and PC9, respectively. This also enabled glycan profiling of the onco-protein EGFR, without immunoprecipitation. Moreover, the glycan specificity afforded by the lectin@MNP allowed as to observe increased terminal and core fucosylation in EGFR from PC9-IR, which demonstrates the potential for protein glycosylation alterations in the resistant cell line. The multi-lectin nanoprobe-based affinity mass spectrometry presents a new method for large-scale glycoproteomic profiling of different sample types without prior fractionation or immunoprecipitation.
HostingRepository	PRIDE
AnnounceDate	2022-02-25
AnnouncementXML	Submission_2022-02-24_17:37:12.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Ju Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-05 04:44:14	ID requested
⏵ 1	2022-02-24 17:37:13	announced
2	2024-10-22 05:33:57	announced	2024-10-22: Updated project metadata.

Waniwan JT, Chen YJ, Capangpangan R, Weng SH, Chen YJ, Glycoproteomic Alterations in Drug-Resistant Nonsmall Cell Lung Cancer Cells Revealed by Lectin Magnetic Nanoprobe-Based Mass Spectrometry. J Proteome Res, 17(11):3761-3773(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Glycoproteom, Human, lung cancer cell, LC-MSMS

Yi-Ju Chen
contact affiliation	Institute of Chemistry, Academia Sinica
contact email	tp7249@gmail.com
lab head
Yi-Ju Chen
contact affiliation	Academia Sinica
contact email	tp7249@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD010035

PRIDE project URI

• PRIDE
  1. PXD010035
     1. Label: PRIDE project
     2. Name: Glycoproteome of Human lung cancer cell by LC-MSMS