PXD009978-1

PXD009978 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization
Description	The Golgi apparatus is the hub of the secretory pathway, trafficking proteins and lipids and synthesizing complex glycans. The spatial distribution of resident proteins across individual Golgi cisternae is functionally essential for complex molecule biosynthesis. However, the inability to-date to separate Golgi cisternae means both the distribution of most residents, and the mechanisms determining their distribution, are unknown. Here, we exploit differences in surface charge between membranes to perform the first separation of Golgi cisternae. We localize over 400 proteins to the Golgi and describe the cisternal distribution of over 250 Golgi residents, as well as complex glycans. Both protein and glycan distributions are validated in-vivo using super-resolution microscopy. Results reveal distinct functional compartmentalization amongst resident Golgi proteins, which relates to the distribution of carbohydrate reaction products. Analysis of cisternal proteomes shows that exoplasmic protein pI, exoplasmic hydrophobicity, Ser content and asymmetric Phe distribution increase across the Golgi.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:15:02.567.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009978
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Harriet Parsons
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-31 03:04:53	ID requested
⏵ 1	2024-10-07 10:15:03	announced

10.6019/PXD009978;

Parsons HT, Stevens TJ, McFarlane HE, Vidal-Melgosa S, Griss J, Lawrence N, Butler R, Sousa MML, Salemi M, Willats WGT, Petzold CJ, Heazlewood JL, Lilley KS, Reveals Underlying Properties of Cisternal Localization. Plant Cell, 31(9):2010-2034(2019) [pubmed]

10.1105/tpc.19.00081;

curator keyword: Biological

submitter keyword: Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization

Harriet T Parsons
contact affiliation	Cambridge University
contact email	tempeparsons@gmail.com
lab head
Harriet Parsons
contact affiliation	Cambridge University
contact email	tempeparsons@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009978
     1. Label: PRIDE project
     2. Name: Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization