PXD009969 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RNA instability in amyotrophic lateral sclerosis |
Description | Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) share key features, including accumulation of the RNA binding protein TDP-43. TDP-43 regulates RNA homeostasis, but it remains unclear whether RNA stability is affected in these disorders. We use Bru-seq and BruChase-seq to assess genome-wide RNA stability in ALS patient-derived cells, demonstrating profound destabilization of ribosomal and mitochondrial transcripts. This pattern is recapitulated by TDP-43 overexpression, suggesting a primary role for TDP-43 in RNA destabilization, and in post-mortem samples from ALS and FTD patients. Proteomics and functional studies illustrate corresponding reductions in mitochondrial components and compensatory increases in protein synthesis. Collectively, these observations suggest that TDP-43 deposition leads to targeted RNA instability in ALS and FTD, and may ultimately cause cell death by disrupting energy production and protein synthesis pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-24 |
AnnouncementXML | Submission_2018-07-24_09:46:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sami Barmada |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-30 15:57:20 | ID requested | |
⏵ 1 | 2018-07-24 09:46:44 | announced | |
2 | 2024-10-22 04:07:37 | announced | 2024-10-22: Updated project metadata. |
Publication List
Tank EM, Figueroa-Romero C, Hinder LM, Bedi K, Archbold HC, Li X, Weskamp K, Safren N, Paez-Colasante X, Pacut C, Thumma S, Paulsen MT, Guo K, Hur J, Ljungman M, Feldman EL, Barmada SJ, Abnormal RNA stability in amyotrophic lateral sclerosis. Nat Commun, 9(1):2845(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: RNA, stability, amyotrophic lateral sclerosis, frontotemporal dementia, stem cells, LC-MS/MS, TMT 10plex |
Contact List
Sami Barmada |
contact affiliation | Assistant Professor of Neurology and Angela Dobson and Lyndon Welch Professor of Neurology Department of Neurology | University of Michigan Health System 109 Zina Pitcher Place, 5015 BSRB | Ann Arbor, MI | 48109 |
contact email | sbarmada@umich.edu |
lab head | |
Sami Barmada |
contact affiliation | University of Michigan |
contact email | sbarmada@umich.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD009969 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009969
- Label: PRIDE project
- Name: RNA instability in amyotrophic lateral sclerosis