PXD009903-1

PXD009903 is an original dataset announced via ProteomeXchange.

Title	Target identification of SB2001 by thermal proteome profiling
Description	In this study, we identified target proteins of SB2001, a cytotoxic agent that acts specifically against HeLa cells. Because SB2001 (= A08) lacked of chemical modification sites, label-free target identification methods using the thermal proteome profiling (TPP) were applied to characterize its mechanism of action. SB2024 (= A11) was used as a negative control compound.
HostingRepository	PRIDE
AnnounceDate	2019-11-04
AnnouncementXML	Submission_2019-11-04_06:36:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hankum Park
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-25 03:45:28	ID requested
⏵ 1	2019-11-04 06:36:12	announced
2	2024-10-22 04:06:42	announced	2024-10-22: Updated project metadata.

Park H, Park SB, Label-free target identification reveals oxidative DNA damage as the mechanism of a selective cytotoxic agent. Chem Sci, 10(12):3449-3458(2019) [pubmed]

curator keyword: Biological

submitter keyword: Thermal proteome profiling, target identification

Seung Bum Park
contact affiliation	Department of Chemistry, Seoul National University
contact email	sbpark@snu.ac.kr
lab head
Hankum Park
contact affiliation	Seoul National University
contact email	hanpark@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD009903

PRIDE project URI

• PRIDE
  1. PXD009903
     1. Label: PRIDE project
     2. Name: Target identification of SB2001 by thermal proteome profiling