PXD009789-1
PXD009789 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | System-wide profiling of protein-RNA interactions in sindbis infected cells reveals host regulators of infection |
Description | RNA viruses rely on cellular RNA-binding proteins (RBPs) to infect the host cell. However, the repertoire of the cellular RBPs exploited by viruses is largely unknown. Using the ‘RNA interactome capture’ method, we profiled in a proteome-wide scale the RBP-RNA interactions occurring during sindbis virus (SINV) infection. We discover that SINV affects the activity of hundreds of RBPs, essentially rewiring the host RNA-bound proteome. Such alterations do not relate to changes in protein abundance but are rather due to subcellular redistribution of RBPs and pervasive transcriptome remodelling. Strikingly, RBPs stimulated by SINV accumulate in the cytoplasmic compartments where the virus replicates and interact with viral RNA. Perturbation of these RBPs can restrict or promote infection; for example, GEMIN5 binds to key regulatory regions of SINV RNAs and inhibits viral protein expression. Importantly, we show that drug based RBP intervention may have potential as therapeutic strategy against viruses. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-28 |
AnnouncementXML | Submission_2019-02-28_09:15:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Caroline Lenz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Sindbis virus; NCBI TaxID: 11034; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-05-15 08:56:55 | ID requested | |
⏵ 1 | 2019-02-28 09:15:27 | announced | |
2 | 2019-03-01 00:30:33 | announced | Updated project metadata. |
Publication List
Garcia-Moreno M, Noerenberg M, Ni S, J, ä, rvelin AI, Gonz, á, lez-Almela E, Lenz CE, Bach-Pages M, Cox V, Avolio R, Davis T, Hester S, Sohier TJM, Li B, Heikel G, Michlewski G, Sanz MA, Carrasco L, Ricci EP, Pelechano V, Davis I, Fischer B, Mohammed S, Castello A, System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection. Mol Cell, 74(1):196-211.e11(2019) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Human, Sindbis virus, LC-MSMS |
Contact List
Alfredo Castello Palomares | |
---|---|
contact affiliation | Department of Biochemistry, University of Oxford, UK |
contact email | alfredo.castellopalomares@bioch.ox.ac.uk |
lab head | |
Caroline Lenz | |
contact affiliation | Department of Biochemistry, University of Oxford |
contact email | caroline.lenz@bioch.ox.ac.uk |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD009789 |
PRIDE project URI |
Repository Record List
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