PXD009745-1

PXD009745 is an original dataset announced via ProteomeXchange.

Title	The activity-dependent bulk endocytosis proteome reveals a key presynaptic role for Rab11
Description	Activity-dependent bulk endocytosis (ADBE) is the dominant mode of synaptic vesicle (SV) endocytosis during high frequency stimulation, suggesting it should play key roles in neurotransmission during periods of intense neuronal activity. However efforts in elucidating the physiological role of ADBE have been hampered by the lack of identified molecules which are unique to this endocytosis mode. To address this, we performed proteomic analysis on purified bulk endosomes, which are a key intermediate in ADBE. Bulk endosomes were enriched via two independent approaches, a classical subcellular fractionation method and isolation via magnetic nanoparticles. There was a 79 % overlap in proteins identified via the two protocols and these molecules formed the ADBE proteome. Bioinformatic analysis revealed a strong enrichment in cell adhesion, cytoskeletal and signalling molecules, in addition to expected SV and trafficking proteins. Network analysis identified rab GTPases as a central hub within the ADBE proteome. Subsequent investigation of a subset of these rabs revealed that constitutively active rab11 both facilitated ADBE and accelerated clathrin-mediated endocytosis. This first result suggests that the ADBE proteome will provide a rich resource for the future study of presynaptic function.
HostingRepository	PRIDE
AnnounceDate	2018-10-15
AnnouncementXML	Submission_2018-10-15_15:18:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-11 00:22:47	ID requested
⏵ 1	2018-10-15 15:18:00	announced
2	2024-10-22 04:45:03	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Dimethyl, Quantitative proteomics, endosomes, nycozend and magnetic nanoparticles

Matthias Trost
contact affiliation	Institute for Cell and molecular Biosciences (ICAMB) Faculty of medical Sciences, Newcastle University Framlington place Newcastle Upon Tyne, NE2 4HH, UK
contact email	matthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009745
     1. Label: PRIDE project
     2. Name: The activity-dependent bulk endocytosis proteome reveals a key presynaptic role for Rab11