PXD009709 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine |
Description | Extracellular vesicles (EVs) have a great potential in clinical applications, however their isolation from different body fluids and their characterisation are not yet optimal or standardised. Here we report the results of examining the performance of ultrafiltration combined with size exclusion chromatography (UF-SEC) to isolate EVs from urine. The results reveal that UF-SEC is a fast method and provides high purity. Furthermore, we introduce asymmetrical-flow field-flow fractionation coupled with a UV detector and multi-angle light-scattering detector (AF4/UV-MALS) as a characterisation method and compare it with the applicabilities and limitations of current methods. We demonstrate that AF4/UV-MALS is a straightforward characterisation method for urinary EVs. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-04 |
AnnouncementXML | Submission_2018-07-04_07:27:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kurt Boonen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-08 02:38:43 | ID requested | |
⏵ 1 | 2018-07-04 07:27:06 | announced | |
2 | 2019-06-17 03:30:29 | announced | Updated publication reference for PubMed record(s): 29988836. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: lc-ms/ms, urine, extracellular vesicle, exosome, orbitrap |
Contact List
Inge Mertens |
contact affiliation | Flemish Institute for Technological Research (VITO) Boeretang 200 2400 Mol |
contact email | inge.mertens@vito.be |
lab head | |
Kurt Boonen |
contact affiliation | University of Antwerp - VITO |
contact email | kurt.boonen@vito.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009709
- Label: PRIDE project
- Name: Ultrafiltration and size exclusion chromatography combined with asymmetrical-flow field-flow fractionation for the isolation and characterisation of extracellular vesicles from urine