PXD009705-2

PXD009705 is an original dataset announced via ProteomeXchange.

Title	Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa
Description	Pseudomonas aeruginosa colonises the upper airway of cystic fibrosis (CF) patients, providing a reservoir of host-adapted genotypes that subsequently establish chronic lung infection. We previously experimentally-evolved P. aeruginosa in a murine model of respiratory tract infection and observed mutations in pmrB that promoted establishment and persistence of infection. Here we show that mutations in pmrB, which encodes the sensor kinase of the PmrAB two-component system, are acquired early in infection and increase resistance to the host antimicrobial lysozyme. Proteomic analysis of pmrB mutants reveal downregulation of proteins involved in LPS biosynthesis, antimicrobial resistance and phenazine production, and upregulation of proteins involved in adherence, lysozyme resistance and inhibition of the chloride ion channel CFTR, relative to wild-type strain LESB65. Accordingly, pmrB mutants show enhanced adherence to airway epithelial cells and downregulate host CFTR expression. P. aeruginosa pmrB mutations are found in CF patient isolates and are associated with the same phenotypes, but are subject to an evolutionary trade-off: enhanced colonisation potential, resistance to host defences and CFTR inhibition, but concomitant increased susceptibility to antibiotics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:52.242.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009705
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-07 06:42:05	ID requested
1	2018-07-11 06:39:56	announced
⏵ 2	2024-10-22 04:45:00	announced	2024-10-22: Updated project metadata.

10.6019/PXD009705;

10.1038/s41467-018-04996-x;

Bricio-Moreno L, Sheridan VH, Goodhead I, Armstrong S, Wong JKL, Waters EM, Sarsby J, Panagiotou S, Dunn J, Chakraborty A, Fang Y, Griswold KE, Winstanley C, Fothergill JL, Kadioglu A, Neill DR, Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa. Nat Commun, 9(1):2635(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Label free quantification,Pseudomonas aeruginosa, pmrB

Julian A. Hiscox
contact affiliation	Chair in Infection and Global Health, Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool,
contact email	julianh@liverpool.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009705
     1. Label: PRIDE project
     2. Name: Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa