PXD009696-2

PXD009696 is an original dataset announced via ProteomeXchange.

Title	Identificatioin of phosphoarginine proteins and sites in Jurkat cells by LC-MS/MS
Description	Acid and heat lability greatly hindered the study of N-phosphorylation (pArg, pHis and pLys), and limited progresses were focus on prokaryotes since the O-phosphorylation is of high abundance in eukaryotes. To meet the challenge, this paper mainly described the identification of phosphoarginine (pArg) proteins and sites in human Jurkat cells by LC-MS/MS under an optimized proteomic experimental procedure. The optimization included cell stimulation conditions, protein extraction methods and MS/MS fragmentation modes. Under this best procedure， hundreds of pArg proteins and sites were unambiguously identified in Jurkat cells from two biological replicates. GO analysis revealed that the pArg proteins may play an important role in nucleic acid-related functions. Besides, this resource also contains the identification information of pHis and pLys.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:13.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Songsen Fu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-07 05:07:36	ID requested
1	2020-07-01 06:28:26	announced
⏵ 2	2024-10-22 05:08:14	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biological

submitter keyword: proteins, phosphoarginine,Jurkat, sites, LC-MS/MS

Yufen Zhao
contact affiliation	Department of Chemical Biology, Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China
contact email	yfzhao@xmu.edu.cn
lab head
Songsen Fu
contact affiliation	Department of Chemical Biology, College of Chemistry and Chemical Engineering, and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University
contact email	fsstswcbyy@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD009696

PRIDE project URI

• PRIDE
  1. PXD009696
     1. Label: PRIDE project
     2. Name: Identificatioin of phosphoarginine proteins and sites in Jurkat cells by LC-MS/MS