PXD009683 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells |
Description | Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the ER but also to the surfaces of lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in SUM159 cells. Rab18 localized to the ER and to LD membranes upon LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover upon cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover, but instead likely plays a specialized role in specific cell types |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-06 |
AnnouncementXML | Submission_2018-06-06_05:34:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zon Lai |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-04 09:00:39 | ID requested | |
⏵ 1 | 2018-06-06 05:34:27 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Lipid droplet, Rab18, RabGTPase, Endoplasmic reticulum, Triacylglycerol, Neutral lipid storage |
Contact List
Tobias Walther |
contact affiliation | Harvard University |
contact email | twalther@hsph.harvard.edu |
lab head | |
Zon Lai |
contact affiliation | Harvard T. H. Chan School of Public Health |
contact email | zlai@hsph.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009683
- Label: PRIDE project
- Name: Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells