PXD009661-1

PXD009661 is an original dataset announced via ProteomeXchange.

Title	Quantitative temporal phosphoproteomics of aging C.elegans under different environmental temperatures
Description	Temperature is one of the primary environmental factors that affect aging, in which protein phosphorylation is an important regulator. Currently, the understanding of phosphorylation events in regulatory networks during aging has remained rather limited. Therefore, the phosphoproteomes of C.elegans of different age groups cultured at 20°C in natural aging process and 25°C in accelerated aging process were analyzed. Through using the iTRAQ-labeled phosphoproteomics method, 2375 phosphoproteins and 9063 phosphosites were identified. Volcano plots illustrated that 208 proteins during natural aging and 130 during accelerated aging, were significantly changed. Gene ontology and pathway analysis revealed that these proteins were mainly involved in temperature response, DNA transcription, protein translation, tissue system development and animal behavior processes. Moreover, our results uncovered those kinases CK2, MAPK and CAMK2 might play important roles in aging regulation. In summary, our results provided a new insight into the complicated phosphor-regulatory network system during aging and an important resource for future studies of protein phosphorylation in worms.
HostingRepository	PRIDE
AnnounceDate	2018-08-08
AnnouncementXML	Submission_2018-08-08_09:32:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jichang Huang
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-03 02:28:23	ID requested
⏵ 1	2018-08-08 09:32:41	announced
2	2024-10-22 04:45:03	announced	2024-10-22: Updated project metadata.

Huang J, Wu Z, Wang J, Zhang X, Quantitative phosphoproteomics reveals GTBP-1 regulating C.elegans lifespan at different environmental temperatures. Biochem Biophys Res Commun, 503(3):1962-1967(2018) [pubmed]

curator keyword: Biological

submitter keyword: C.elegans

phosphoproteomics

iTRAQ

natural aging

accelerated aging

Xumin Zhang
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200438, China
contact email	xumin_zhang@fudan.edu.cn
lab head
Jichang Huang
contact affiliation	School of Life Sciences, Fudan University
contact email	14110700074@fudan.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009661
     1. Label: PRIDE project
     2. Name: Quantitative temporal phosphoproteomics of aging C.elegans under different environmental temperatures