PXD009649-1

PXD009649 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the Ade6-M26 meiotic recombination hotspot in the fission yeast schizosaccharomyces pombe
Description	Homologous recombination events during meiosis cluster at specific loci called hotspots. Hotspot activation requires accessible chromatin, DNA sequence motifs DNA sequence-specific binding proteins, but how these proteins create a suitable environment for Rec12 recruitment is unknown. Here, we purified the ade6-M26 meiotic recombination hotspot from Schizosaccharomyces pombe and identified novel regulators of hotspot activation by mass spectrometry. Small, circular minichromosomes containing the ade6-M26 hotspot or control allele were transformed into strains permitting synchronous meiotic induction and purified from these cultures at sequential time points. Enrichment of known M26 binding factors Atf1 and Pcr1 in the data set validated the approach. Filtering for chromatin-related proteins enriched in the hotspot revealed candidate hotspot regulators, which were validated using genetic studies. This study highlights the power of unbiased proteomic screens to reveal novel insights into fundamental biological processes, and highlights a critical role in H2AZ turnover in mediating access of transcription factors to their underlying DNA-sequence motifs.
HostingRepository	PRIDE
AnnounceDate	2018-11-04
AnnouncementXML	Submission_2018-11-04_14:45:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009649
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aaron Storey
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	Methyl; Dimethyl; Oxidation; Acetyl; Carbamidomethyl; Trimethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-02 10:41:06	ID requested
⏵ 1	2018-11-04 14:45:54	announced

Storey AJ, Wang HP, Protacio RU, Davidson MK, Tackett AJ, Wahls WP, Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot. Epigenetics Chromatin, 11(1):64(2018) [pubmed]

curator keyword: Biological

submitter keyword: meiotic recombination hotspot m26 affinity purification

Wayne P Wahls
contact affiliation	Professor of Biochemistry and Molecular Biology University of Arkansas for Medical Sciences
contact email	WahlsWayneP@uams.edu
lab head
Aaron Storey
contact affiliation	University of Arkansas for Medical Sciences
contact email	ajstorey@uams.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009649
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the Ade6-M26 meiotic recombination hotspot in the fission yeast schizosaccharomyces pombe