PXD009645-2

PXD009645 is an original dataset announced via ProteomeXchange.

Title	Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona
Description	The increasing number of biological applications for black phosphorus (BP) nanomaterials has precipitated considerable concern about their interactions with physiological systems. Here, we demonstrate the adsorption of plasma protein onto BP nanomaterials and the subsequent immune perturbation effect on macrophages. Using liquid chromatography tandem mass spectrometry, we discovered that BP nanosheets (BPNSs) were able to bind 23.3 percent of immune proteins from plasma, while BP quantum dots (BPQDs) bound 41.8 percent of immune proteins. In particular, the protein corona dramatically reshaped BP nanomaterial-corona complexes, influenced cellular uptake, activated the NF-κB pathway and even increased cytokine secretion by 2-5-fold. BP nanomaterials induced immunotoxicity and immune perturbation in macrophages in the presence of a plasma corona. These findings offer important insights into the development of safe and effective BP nanomaterial-based therapies.
HostingRepository	PRIDE
AnnounceDate	2018-05-18
AnnouncementXML	Submission_2018-07-02_07:23:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianbin Mo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-02 03:30:33	ID requested
1	2018-05-18 06:27:03	announced
⏵ 2	2018-07-02 07:23:25	announced	Updated publication reference for PubMed record(s): 29946125.
3	2024-10-22 04:45:29	announced	2024-10-22: Updated project metadata.

Mo J, Xie Q, Wei W, Zhao J, Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona. Nat Commun, 9(1):2480(2018) [pubmed]

curator keyword: Biological

submitter keyword: black phosphorus nanomaterials, corona, human plasma, LC-MS/MS

Jing Zhao
contact affiliation	State Key Laboratory of Coordination Chemistry, Institute of Chemistry and BioMedical Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
contact email	jingzhao@nju.edu.cn
lab head
Jianbin Mo
contact affiliation	Nanjing University
contact email	492704926@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD009645

PRIDE project URI

• PRIDE
  1. PXD009645
     1. Label: PRIDE project
     2. Name: Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona