PXD009640-2

PXD009640 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis SNAREs SYP121 and SYP122 mediate the secretion of distinct cargo 2 subsets
Description	SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins drive vesicle fusion and contribute to homoeostasis, pathogen defence, cell expansion, and growth in plants. In Arabidopsis thaliana, the two homologous Qa-SNAREs, SYP121 and SYP122 facilitate the majority of secretory traffic to the plasma membrane. In the absence of stress, the single mutants are indistinguishable from wild-type plants, implying a redundancy in their functions. Nonetheless, several studies suggest differences among the secretory cargo of these SNAREs. To address this issue, we conducted an analysis of the proteins secreted by cultured wild-type, syp121 and syp122 mutant Arabidopsis seedlings. Here, we report that a number of cargo proteins are associated differentially with traffic mediated by SYP121 and SYP122. The data also point to important overlaps between the SNAREs. The findings lead us to conclude that the two Qa-SNAREs mediate distinct, but complementary secretory pathways during vegetative plant growth.
HostingRepository	PRIDE
AnnounceDate	2018-09-18
AnnouncementXML	Submission_2018-11-21_06:11:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009640
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alex Jones
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-01 06:17:24	ID requested
1	2018-09-18 07:58:52	announced
⏵ 2	2018-11-21 06:11:33	announced	Updated publication reference for PubMed record(s): 30348815.

Waghmare S, Lileikyte E, Karnik R, Goodman JK, Blatt MR, Jones AME, SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets. Plant Physiol, 178(4):1679-1688(2018) [pubmed]

curator keyword: Biological

submitter keyword: Secreted proteins, syntaxin, SNARE, proteomics, cell wall, apoplast.

Alexandra M. E. Jones
contact affiliation	University of Warwick, Shool of Life Sciences and Proteomics Research Technology Platform.
contact email	alex.jones@warwick.ac.uk
lab head
Alex Jones
contact affiliation	University of Warwick
contact email	alex.jones@warwick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009640
     1. Label: PRIDE project
     2. Name: Arabidopsis SNAREs SYP121 and SYP122 mediate the secretion of distinct cargo 2 subsets