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PXD009581-1

PXD009581 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proteome-wide screen of Cdc14 interactors in Candida albicans
DescriptionThe Cdc14 phosphatase plays a key role in organising the intricate choreography of mitosis and cell division. In order to understand the role of Cdc14 in the human fungal pathogen Candida albicans we used quantitative proteomics to identify interacting proteins of CaCdc14. We used an orthogonal approach of a substrate trapping mutant combined with affinity purification-mass spectrometry analysis using stable isotope labelling in cell culture (SILAC). The results identified 126 proteins that interact with Cdc14 of which 80% are novel. These provide further insight into the function of Cdc14, highlighting roles in regulating the attachment of the mitotic spindle to kinetochores, mitotic exit, cytokinesis, licensing of DNA replication by re-activating pre-replication complexes, and DNA repair. Five Cdc14-interacting proteins with previously unknown functions localized to the Spindle Pole Bodies (SPBs). Intriguingly, there was suggestive evidence that Cdc14 substrates may include components of the ergosterol biosynthesis pathway targeted by azole anti-fungal drugs Thus we have greatly expanded the set of known substrates of Cdc14 by which it carries out these roles.
HostingRepositoryPRIDE
AnnounceDate2019-11-12
AnnouncementXMLSubmission_2019-11-12_11:06:35.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIliyana Kaneva
SpeciesList scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationListmonohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-24 01:43:29ID requested
12019-11-12 11:06:36announced
22024-10-22 04:44:42announced2024-10-22: Updated project metadata.
Publication List
Kaneva IN, Sudbery IM, Dickman MJ, Sudbery PE, Proteins that physically interact with the phosphatase Cdc14 in Candida albicans have diverse roles in the cell cycle. Sci Rep, 9(1):6258(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Cdc14, Candida albicans, SILAC, nativeSILAC
Contact List
Mark Dickman
contact affiliationDepartment of Chemical and Biological Engineering, University of Sheffield
contact emailm.dickman@sheffield.ac.uk
lab head
Iliyana Kaneva
contact affiliationUniversity of Sheffield
contact emaililiyana.kaneva@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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