PXD009581-1

PXD009581 is an original dataset announced via ProteomeXchange.

Title	A proteome-wide screen of Cdc14 interactors in Candida albicans
Description	The Cdc14 phosphatase plays a key role in organising the intricate choreography of mitosis and cell division. In order to understand the role of Cdc14 in the human fungal pathogen Candida albicans we used quantitative proteomics to identify interacting proteins of CaCdc14. We used an orthogonal approach of a substrate trapping mutant combined with affinity purification-mass spectrometry analysis using stable isotope labelling in cell culture (SILAC). The results identified 126 proteins that interact with Cdc14 of which 80% are novel. These provide further insight into the function of Cdc14, highlighting roles in regulating the attachment of the mitotic spindle to kinetochores, mitotic exit, cytokinesis, licensing of DNA replication by re-activating pre-replication complexes, and DNA repair. Five Cdc14-interacting proteins with previously unknown functions localized to the Spindle Pole Bodies (SPBs). Intriguingly, there was suggestive evidence that Cdc14 substrates may include components of the ergosterol biosynthesis pathway targeted by azole anti-fungal drugs Thus we have greatly expanded the set of known substrates of Cdc14 by which it carries out these roles.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_11:06:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iliyana Kaneva
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-24 01:43:29	ID requested
⏵ 1	2019-11-12 11:06:36	announced
2	2024-10-22 04:44:42	announced	2024-10-22: Updated project metadata.

Kaneva IN, Sudbery IM, Dickman MJ, Sudbery PE, Proteins that physically interact with the phosphatase Cdc14 in Candida albicans have diverse roles in the cell cycle. Sci Rep, 9(1):6258(2019) [pubmed]

curator keyword: Biological

submitter keyword: Cdc14, Candida albicans, SILAC, nativeSILAC

Mark Dickman
contact affiliation	Department of Chemical and Biological Engineering, University of Sheffield
contact email	m.dickman@sheffield.ac.uk
lab head
Iliyana Kaneva
contact affiliation	University of Sheffield
contact email	iliyana.kaneva@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD009581

PRIDE project URI

• PRIDE
  1. PXD009581
     1. Label: PRIDE project
     2. Name: A proteome-wide screen of Cdc14 interactors in Candida albicans