PXD009581 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A proteome-wide screen of Cdc14 interactors in Candida albicans |
Description | The Cdc14 phosphatase plays a key role in organising the intricate choreography of mitosis and cell division. In order to understand the role of Cdc14 in the human fungal pathogen Candida albicans we used quantitative proteomics to identify interacting proteins of CaCdc14. We used an orthogonal approach of a substrate trapping mutant combined with affinity purification-mass spectrometry analysis using stable isotope labelling in cell culture (SILAC). The results identified 126 proteins that interact with Cdc14 of which 80% are novel. These provide further insight into the function of Cdc14, highlighting roles in regulating the attachment of the mitotic spindle to kinetochores, mitotic exit, cytokinesis, licensing of DNA replication by re-activating pre-replication complexes, and DNA repair. Five Cdc14-interacting proteins with previously unknown functions localized to the Spindle Pole Bodies (SPBs). Intriguingly, there was suggestive evidence that Cdc14 substrates may include components of the ergosterol biosynthesis pathway targeted by azole anti-fungal drugs Thus we have greatly expanded the set of known substrates of Cdc14 by which it carries out these roles. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-12 |
AnnouncementXML | Submission_2019-11-12_11:06:35.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Iliyana Kaneva |
SpeciesList | scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-24 01:43:29 | ID requested | |
⏵ 1 | 2019-11-12 11:06:36 | announced | |
2 | 2024-10-22 04:44:42 | announced | 2024-10-22: Updated project metadata. |
Publication List
Kaneva IN, Sudbery IM, Dickman MJ, Sudbery PE, Proteins that physically interact with the phosphatase Cdc14 in Candida albicans have diverse roles in the cell cycle. Sci Rep, 9(1):6258(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Cdc14, Candida albicans, SILAC, nativeSILAC |
Contact List
Mark Dickman |
contact affiliation | Department of Chemical and Biological Engineering, University of Sheffield |
contact email | m.dickman@sheffield.ac.uk |
lab head | |
Iliyana Kaneva |
contact affiliation | University of Sheffield |
contact email | iliyana.kaneva@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009581
- Label: PRIDE project
- Name: A proteome-wide screen of Cdc14 interactors in Candida albicans