PXD009569-2

PXD009569 is an original dataset announced via ProteomeXchange.

Title	Immunoproteomics of Lymph Node T-cells in Bladder Cancer
Description	The immune-suppressive features often possessed by invasive tumors hamper effective anti-tumor immunity. In this context, tumor-infiltrating regulatory T cells (Tregs) have been widely implied, principally as unwanted suppressors of anti-tumor immune responses. However, while many studies have focused on tumor-infiltrating Tregs, the function and signaling of Tregs in tumor-associated lymph nodes is largely unknown. In this study, we set out to clarify how immune signaling in lymph nodes is impacted by its contact to the tumour. Because muscle-invasive urothelial bladder cancer (MIBC) represent an immunogenic cancer were Tregs are accumulated and sentinel nodes (SNs) can be efficiently detected, we explored the protein expression of T-cells in SNs and non-SNs of MIBC patients. Proteomic analysis of Tregs and effector T-cells in SN and non-draining lymph nodes found SN-Tregs in MIBC patients to up-regulate growth and immune signaling pathways, the cytokine IL-16 being central in the signaling network. Experimental validation showed that in Tregs, tumoral factors increase IL-16 processing into bioactive forms and increase FOXP3 expression. In conclusion, altered IL-16 processing caused by tumour-released factors stimulate expansion of SN-Tregs in MIBC, creating an immunosuppressive environment and contributing to immune escape.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:12:05.037.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Mints
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-23 02:00:45	ID requested
1	2020-09-08 23:04:19	announced
⏵ 2	2024-10-22 05:12:09	announced	2024-10-22: Updated project metadata.

10.1111/sji.12926;

Krantz D, Mints M, Winerdal M, Riklund K, Rutishauser D, Zubarev R, Zirakhzadeh AA, Alamdari F, Johansson M, Sherif A, Winqvist O, IL-16 processing in sentinel node regulatory T cells is a factor in bladder cancer immunity. Scand J Immunol, 92(6):e12926(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Lymph Nodes, T-cells, Cancer

Ola Winqvist
contact affiliation	Karolinska Institutet, Department of Medicine Solna, Unit of Translational Immunology
contact email	ola.winqvist@ki.se
lab head
Michael Mints
contact affiliation	Umeå University
contact email	michael.mints@umu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD009569

PRIDE project URI

• PRIDE
  1. PXD009569
     1. Label: PRIDE project
     2. Name: Immunoproteomics of Lymph Node T-cells in Bladder Cancer