PXD009547-1
PXD009547 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A novel differential ion mobility interface expands the depth of proteome coverage and the sensitivity of multiplex proteomic measurements |
| Description | We present a new high field asymmetric waveform ion mobility spectrometry (FAIMS) interface that can be coupled to the Orbitrap Tribrid mass spectrometers. The interface provides several advantages over previous generations of FAIMS devices including ease of operation, robustness, and high ion transmission. Replicate LC-FAIMS-MS/MS analyses (N=100) of protein digests showed stable ion current over extended time periods with uniform peptide identification on more than 10,000 distinct peptides. For complex tryptic digest analyses, the coupling of FAIMS to LC-MS/MS enabled a 30% gain in unique peptide identification compared to non FAIMS experiments. Improvement in sensitivity facilitated the identification of low abundance peptides, and extended the limit of detection by almost an order of magnitude. The reduction in chimeric MS/MS spectra using FAIMS also improved the precision and the number of quantifiable peptides when using isobaric labeling with tandem mass tag (TMT) 10-plex reagent. We compared quantitative proteomic measurements for LC-MS/MS analyses performed using synchronous precursor selection (SPS) and LC-FAIMS-MS/MS to profile the temporal changes in protein abundance of HEK293 cells following heat shock for periods up to 9h. FAIMS provided 2.5-fold increase in the number of quantifiable peptides compared to non-FAIMS. Altogether, the enhancement in ion transmission and duty cycle of the new FAIMS interface extended the depth and comprehensiveness of proteomic analyses and improved the precision of quantitative measurements. |
| HostingRepository | MassIVE |
| AnnounceDate | 2018-07-31 |
| AnnouncementXML | Submission_2018-07-31_09:38:08.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Francis McManus |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho; Deamidated; Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-04-19 09:02:57 | ID requested | |
| ⏵ 1 | 2018-07-31 09:38:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: High field asymmetric waveform ion mobility spectrometry (FAIMS), quantitative proteomics, isobaric peptide labeling, tandem mass tag (TMT), heat shock |
Contact List
| Pierre Thibault | |
|---|---|
| contact affiliation | University of Montreal |
| contact email | pierre.thibault@umontreal.ca |
| lab head | |
| Francis McManus | |
| contact affiliation | University of Montreal |
| contact email | francis.mcmanus@umontreal.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082293 |
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