PXD009536-2

PXD009536 is an original dataset announced via ProteomeXchange.

Title	Neuropeptidomic Analysis of the Stick Insect, Carausius morosus
Description	One of the most thoroughly studied insect species, with respect to locomotion behaviour, is the stick insect Carausius morosus. Although detailed information exists on premotor networks controlling walking, surprisingly little is known about neuropeptides, which are certainly involved in motor activity generation and modulation. So far, only few neuropeptides were identified from C. morosus or related stick insects. We performed a transcriptome analysis of the central nervous system to assemble and identify 65 neuropeptide and protein hormone precursors of C. morosus, including five novel putative neuropeptide precursors without clear homology to known neuropeptide precursors of other insects (Carausius neuropeptide-like precursor 1, HanSolin, PK-like1, PK-like2, RFLamide). Using Q Exactive Orbitrap and MALDI-TOF mass spectrometry, 277 peptides including 153 likely bioactive mature neuropeptides were confirmed. Peptidomics yielded a complete coverage for many of the neuropeptide propeptides and confirmed a surprisingly high number of heterozygous sequences. Few neuropeptide precursors commonly occurring in insects, including those of insect kinins and sulfakinins, could neither be found in the transcriptome data nor did peptidomics support their presence. The results of our study represent one of the most comprehensive peptidomic analyses on insects and provide the necessary input for subsequent experiments revealing neuropeptide function in greater detail.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:20.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sander Liessem
SpeciesList	scientific name: Carausius morosus; NCBI TaxID: 7022;
ModificationList	mannosylated residue; L-cystine (cross-link); 2-pyrrolidone-5-carboxylic acid (Glu); amidated residue; methylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-19 01:07:44	ID requested
1	2018-04-30 01:49:15	announced
⏵ 2	2024-10-22 04:44:26	announced	2024-10-22: Updated project metadata.

Liessem S, Ragionieri L, Neupert S, B, ü, schges A, Predel R, Transcriptomic and Neuropeptidomic Analysis of the Stick Insect, Carausius morosus. J Proteome Res, 17(6):2192-2204(2018) [pubmed]

10.1021/acs.jproteome.8b00155;

curator keyword: Biological

submitter keyword: peptidomics, stick insects, mass spectrometry, neurohormones, nervous system, neuropeptide precursors,Carausius morosus

Reinhard Predel
contact affiliation	Department for Biology, Institute for Zoology, University of Cologne
contact email	rpredel@uni-koeln.de
lab head
Sander Liessem
contact affiliation	Department for Biology, Institute for Zoology, University of Cologne, Predel Lab
contact email	sliessem@smail.uni-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009536
     1. Label: PRIDE project
     2. Name: Neuropeptidomic Analysis of the Stick Insect, Carausius morosus