PXD009460-4
PXD009460 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of disulfide linkages in proteins by 193 nm ultraviolet photodissociation (UVPD) |
| Description | Inter-linked disulfide bonds connecting peptide chains are homolytically cleaved with 193 nm ultraviolet photodissociation (UVPD). Analysis of insulin demonstrates the ability for UVPD to cleave multiple disulfide bonds and provide sequence coverage of multiple peptide chains in the same MS/MS event. The information provided by UVPD of disulfide-bound peptides is comparable to information garnered from other high energy dissociation methods but requires an activation period an order of magnitude faster than these methods. This phenomenon was investigated, along with a partial reduction and alkylation sample preparation to determine disulfide connectivities of enzymatically digested proteins. The 4 disulfide bonds of lysozyme and the 19 disulfide bonds of serotransferrin were unambiguously characterized through non-reduced and partially reduced protein digestions. |
| HostingRepository | jPOST |
| AnnounceDate | 2018-04-28 |
| AnnouncementXML | Submission_2021-02-04_17:27:16.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | M. Montana Quick |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Gallus gallus; NCBI TaxID: 9031; scientific name: Bos taurus; NCBI TaxID: 9913; |
| ModificationList | unknown modification; N-ethylmaleimide derivatized cysteine |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-04-10 13:18:06 | ID requested | |
| 1 | 2018-04-27 10:25:08 | announced | |
| 2 | 2018-09-19 00:54:35 | announced | Updated CV, FTP location and Modification list. |
| 3 | 2019-10-09 17:03:25 | announced | 2019-10-10: Updated PubMed. |
| ⏵ 4 | 2021-02-04 17:27:17 | announced | 2021-02-04: Updated FTP location. |
| 5 | 2022-09-18 02:48:34 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Quick MM, Crittenden CM, Rosenberg JA, Brodbelt JS, Characterization of Disulfide Linkages in Proteins by 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry. Anal Chem, 90(14):8523-8530(2018) [pubmed] |
Keyword List
| submitter keyword: Disulfide bond, Mass spectrometry, ultraviolet photodissociation, UVPD, HPLC |
Contact List
| Jennifer S. Brodbelt | |
|---|---|
| lab head | |
| M. Montana Quick | |
| contact affiliation | University of Texas at Austin |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000407 |
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