PXD009451-1

PXD009451 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics identification of seminal fluid proteins in male Drosophila melanogaster
Description	Seminal fluid contains some of the fastest evolving proteins currently known. These seminal fluid proteins (Sfps) play crucial roles in reproduction, such as supporting sperm function, and – particularly in insects – modifying female physiology and behaviour. Identification of Sfps in small animals is challenging, and often relies on samples taken from the female reproductive tract after mating. A key pitfall of this method is that it might miss Sfps that are of low abundance due to dilution in the female-derived sample or rapid processing in females. Here we present a new and complimentary method, which provides added sensitivity to Sfp identification. We applied label-free quantitative proteomics to Drosophila melanogaster male reproductive tissue – where Sfps are unprocessed, and highly abundant – and quantified Sfps before and immediately after mating, to infer those transferred during copulation. We also analysed female reproductive tracts immediately before and after copulation to confirm the presence and abundance of known and candidate Sfps, where possible. Results were cross-referenced with transcriptomic and sequence databases to improve confidence in Sfp detection. Our data was consistent with 124 previously reported Sfps. We found 8 high-confidence novel candidate Sfps, which were both depleted in mated versus unmated males and identified within the reproductive tract of mated but not virgin females. We also identified 31 more candidates that are likely Sfps based on their abundance, known expression and predicted characteristics, and revealed that four proteins previously identified as Sfps are at best minor contributors to the ejaculate. The estimated copy numbers for our candidate Sfps were lower than for previously identified Sfps, supporting the idea that our technique provides a deeper analysis of the Sfp proteome than previous studies. Our results demonstrate a novel, high-sensitivity approach to the analysis of seminal fluid proteomes, whose application will further our understanding of reproductive biology.
HostingRepository	PRIDE
AnnounceDate	2018-12-19
AnnouncementXML	Submission_2018-12-19_08:32:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Charles
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-10 03:01:00	ID requested
⏵ 1	2018-12-19 08:32:57	announced
2	2024-10-22 04:48:31	announced	2024-10-22: Updated project metadata.

Sepil I, Hopkins BR, Dean R, Th, é, z, é, nas ML, Charles PD, Konietzny R, Fischer R, Kessler BM, Wigby S, . Mol Cell Proteomics, 18(Suppl 1):S46-S58(2019) [pubmed]

submitter keyword: Drosophila melanogaster

Seminal fluid

LFQ

Stuart Wigby
contact affiliation	Edward Grey Institute, Department of Zoology, University of Oxford, Oxford, UK
contact email	stuart.wigby@zoo.ox.ac.uk
lab head
Philip Charles
contact affiliation	University of Oxford
contact email	philip.charles@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD009451

PRIDE project URI

• PRIDE
  1. PXD009451
     1. Label: PRIDE project
     2. Name: Quantitative proteomics identification of seminal fluid proteins in male Drosophila melanogaster