PXD009443 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Temporal profiling of redox-dependent heterogeneity in single cells |
Description | Cellular redox status affects diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus, individual differences in redox status can give rise to distinct sub-populations even among cells with identical genetic backgrounds. Here, we have created a novel methodology to track redox status at single cell resolution using the redox-sensitive probe roGFP. Our method allows identification and sorting of sub-populations with different oxidation levels in either the cytosol, mitochondria or peroxisomes. Using this approach we defined redox-dependent heterogeneity of yeast cells, and characterized growth, as well as proteomic and transcriptomic profiles of subpopulations of cells that differ in their redox status, but are similar in age. We report that, starting in late logarithmic growth, cells of the same age have a bi-modal distribution of oxidation status. A comparative proteomic analysis between these populations identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal oxidation levels and may serve as first line of defense proteins in redox homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-18 |
AnnouncementXML | Submission_2018-06-18_00:54:40.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Meytal Radzinski |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetic acid derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-09 06:15:15 | ID requested | |
⏵ 1 | 2018-06-18 00:54:41 | announced | |
2 | 2018-06-18 05:24:34 | announced | Updated publication reference for PubMed record(s): 29869985. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: redox, yeast |
Contact List
Dr. Dana Reichmann |
contact affiliation | Dept. of Biological Chemistry The Alexander Silberman Institute of Life Science The Hebrew University of Jerusalem |
contact email | danare@mail.huji.ac.il |
lab head | |
Meytal Radzinski |
contact affiliation | Hebrew University of Jerusalem |
contact email | meytal.radzinski@mail.huji.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009443
- Label: PRIDE project
- Name: Temporal profiling of redox-dependent heterogeneity in single cells