PXD009440-1

PXD009440 is an original dataset announced via ProteomeXchange.

Title	Human Salivary Prolactine Inducible Protein (PIP)
Description	In the HPLC high-resolution ESI-MS profiles of both multiple sclerosis subjects and healthy controls saliva samples three proteins, with exp. monoisotopic ion [M+H]+ at 13493.9 ± 0.2 m/z, 13696.9 ± 0.2 m/z (+203 Da, with respect to 13493.9), and 13843.1 ± 0.2 m/z (+147 Da, with respect to 13696.9) eluting between 37.8-38.2 minutes, were detected. The mass difference of 203 Da suggested that the protein with exp. monoisotopic [M+H]+ at 13696.9 ± 0.2 m/z could correspond to the N-acetylhexosamine (theor. monoisotopic ion at 203.1 m/z) derivative of the 13493.9 ± 0.2 m/z protein. On the other hand, the mass difference of 146 Da between the proteins with exp. monoisotopic [M+H]+ at 13843.1 ± 0.2 m/z and 13696.9 ± 0.2 m/z was in agreement with an additional deoxyhesose moiety (theor. monoisotopic ion at 146.1 m/z). Manual inspection of the high-resolution MS/MS spectra of the [M+10H]+10 ions at 1351.00, 1371.32 and 1386.11 m/z allowed to establish that the three proteins were different proteoforms of PIP with the N-terminal glutamine residue converted to pyro-glutamic acid and with two disulfide bonds, but not to assign the glycosylation site. The presence in the MS/MS spectra of low-molecular weight saccharide oxoniun ions at 204.087 and 138.106 m/z confirmed the presence of an N-acetylhexosamine moiety in the protein with [M+H]+ at 13696.9 ± 0.2 m/z.
HostingRepository	PRIDE
AnnounceDate	2018-08-10
AnnouncementXML	Submission_2018-08-10_06:30:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Manconi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); N-acetylhexosaminylated; disulfide crosslinked residues; deoxyhexosylated
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-09 03:14:34	ID requested
⏵ 1	2018-08-10 06:30:06	announced
2	2024-10-22 04:44:35	announced	2024-10-22: Updated project metadata.

Manconi B, Liori B, Cabras T, Vincenzoni F, Iavarone F, Lorefice L, Cocco E, Castagnola M, Messana I, Olianas A, Top-down proteomic profiling of human saliva in multiple sclerosis patients. J Proteomics, 187():212-222(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, saliva, LTQ Orbitrap

Barbara Manconi
contact affiliation	Dip. Scienze della Vita e dell'Ambiente (DISVA) Macrosezione Biomedica - laboratorio di Biochimica Università degli Studi di Cagliari
contact email	bmanconi@unica.it
lab head
Barbara Manconi
contact affiliation	University of Cagliari
contact email	bmanconi@unica.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009440
     1. Label: PRIDE project
     2. Name: Human Salivary Prolactine Inducible Protein (PIP)