PXD009426 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sample preparation for proteomics |
Description | Urea-containing buffer solutions are generally used in proteomic studies to aid protein denaturation and solubilisation during cell and tissue lysis. It is well-known, however, that urea can lead to the carbamylation of peptides and proteins and; subsequently, incomplete digestion of proteins. In our work, we sought for ways to retain urea in the digestion protocol to reduce sample handling, while minimizing urea-related drawbacks. |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-06 |
AnnouncementXML | Submission_2018-06-06_00:02:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lazaro Hiram Betancourt Nunez |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; |
ModificationList | carbamoylated residue; monohydroxylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-06 03:41:07 | ID requested | |
⏵ 1 | 2018-06-06 00:02:07 | announced | |
Publication List
Betancourt LH, Sanchez A, Pla I, Kuras M, Zhou Q, Andersson R, Marko-Varga G, C. J Proteome Res, 17(7):2556-2561(2018) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: urea, LC-MS/MS, Lys-C/trypsin |
Contact List
Gyorgy Marko-Varga |
contact affiliation | Div. Clinical Protein Science & Imaging Dept. of Clinical Sciences (Lund) and Dept. of Biomedical Engineering Lund University |
contact email | gyorgy.marko-varga@bme.lth.se |
lab head | |
Lazaro Hiram Betancourt Nunez |
contact affiliation | Lund University |
contact email | lazaro.betancourt@med.lu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009426 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009426
- Label: PRIDE project
- Name: Sample preparation for proteomics