PXD009389-1

PXD009389 is an original dataset announced via ProteomeXchange.

Title	Shotgun Proteomics and Quantitative Pathway Analysis Study of the Mechanisms of Action of Dehydroeffusol, a Bioactive Phyto-chemical in Medulla Junci with Anti-cancer Activity
Description	Dehydroeffusol (DHE) is a rare compound of phenanthrene class isolated from Medulla Junci used in Traditional Chinese herbal medicines. Initial biological evaluation of this compound reveals in vitro and in vivo anticancer ac-tivities. We performed a shotgun proteomic analysis using Orbitrap liquid chromatography-tandem mass spectrometry to investigate the changes in the protein profiles in cancer cells upon DHE treatment. DHE affected cancer-associated signaling pathways, including NF-B, β-catenin and endoplasmic reticulum stress. Through quantitative pathway and key-node analysis of the proteomics data, activating transcription factor 2 (ATF-2) and c-Jun kinase (JNK) were found to be the key components in DHE’s modulated biological pathways. Furthermore, based on the pathway analysis as well as chemical similarity, DHE is predicted to be a phytoestrogen. Our predictions derived from proteomic, bio-informatic and chemoinformatic analyses were validated with individual cell-based experiments. In addition to elucidating the major mechanisms of action of DHE, our study demonstrates an effective workflow for identifying the mechanisms of action of bioactive compounds through shotgun proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2018-06-05
AnnouncementXML	Submission_2018-06-05_07:12:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Man Eva Fung
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; deaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-04 04:03:59	ID requested
⏵ 1	2018-06-05 07:12:23	announced

Chang IS, Sy LK, Cao B, Lum CT, Kwong WL, Fung YE, Lok CN, Che CM, Shotgun Proteomics and Quantitative Pathway Analysis of the Mechanisms of Action of Dehydroeffusol, a Bioactive Phytochemical with Anticancer Activity from Juncus effusus. J Proteome Res, 17(7):2470-2479(2018) [pubmed]

curator keyword: Biological

submitter keyword: Shotgun proteomics, signaling pathway analysis,TMT-labeling, phytoestrogen

Chi-Ming Che
contact affiliation	The University of Hong Kong, Department of Chemistry, Chemical Biology Centre and State Key Laboratory of Synthetic Chemistry
contact email	cmche@hku.hk
lab head
Yi Man Eva Fung
contact affiliation	The University of Hong Kong
contact email	eva.fungym@hku.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009389
     1. Label: PRIDE project
     2. Name: Shotgun Proteomics and Quantitative Pathway Analysis Study of the Mechanisms of Action of Dehydroeffusol, a Bioactive Phyto-chemical in Medulla Junci with Anti-cancer Activity