PXD009377-2

PXD009377 is an original dataset announced via ProteomeXchange.

Title	Dataset in support of Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite-host cross talk
Description	The highly prevalent gut helminth, Ascaris suum, compromise pigs health and reduce farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people and causes approximately 1.31 million disability-adjusted life years. The humaninfections are often chronic by nature and the parasites have a profound ability to modulate their hosts immune responses. This study provides the first in-depth characterization of extracellular vesicles (EVs) from different developmental stages and body parts of A. suum and their potential role in the host-parasite interplay. The release of EVs during the third larval stage (L3), L4 and adults was demonstrated by Transmission Electron Microscopy, and the uptake of EVs from adult A. suum in intestinal epithelial cells followed by accumulation of RNA in the nucleus by confocal microscopy. Next Generation Sequencing of EV-derived mi/m?RNA identified a number of micro(mi)RNAs from the different A. suum life stages and body parts and potential transcripts of potential host immune targets, such as IL-13, IL-25 and IL-33, were identified. Proteomics of EVs identified several proteins with immunomodulatory properties and other proteins previously shown to be associated with parasite EVs. Furthermore, EVs from A. suum body fluid stimulated the production of the pro-inflammatory cytokines IL-6 and TNF-α in dendritic cells in vitro. Taken together, these results suggest that A. suum EVs and their cargo may play a role in host-parasite interactions. This knowledge may pave the way for novel strategies for helminth infection control and knowledge of their immune modulatory role.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:15.907.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Allan Stensballe
SpeciesList	scientific name: Ascaris suum; NCBI TaxID: 6253;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-03 08:13:49	ID requested
1	2019-11-12 10:45:38	announced
⏵ 2	2024-10-22 04:44:24	announced	2024-10-22: Updated project metadata.

10.1080/20013078.2019.1578116;

Hansen EP, Fromm B, Andersen SD, Marcilla A, Andersen KL, Borup A, Williams AR, Jex AR, Gasser RB, Young ND, Hall RS, Stensballe A, Ovchinnikov V, Yan Y, Fredholm M, Thamsborg SM, Nejsum P, provides evidence of parasite-host cross talk. J Extracell Vesicles, 8(1):1578116(2019) [pubmed]

curator keyword: Biological

submitter keyword: proteomics, miRNA, extracellular vesicles,Ascaris suum, immunity, host-parasite interactions

Allan Stensballe
contact affiliation	Laboratory for Medical Massspectroemtry Aalborg University
contact email	as@hst.aau.dk
lab head
Allan Stensballe
contact affiliation	Aalborg University
contact email	as@hst.aau.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD009377

PRIDE project URI

• PRIDE
  1. PXD009377
     1. Label: PRIDE project
     2. Name: Dataset in support of Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite-host cross talk