PXD009366-2

PXD009366 is an original dataset announced via ProteomeXchange.

Title	protein-protein interaction dataset TNFR1-mediated NFkB signaling
Description	Most MS-based approaches to study protein-protein interactions require homogenization of the cells, which leads to loss of the cellular structures, dilution of proteins and formation of incorrect protein complexes. Furthermore, several purification and washing steps are needed, which can lead to the loss of (weaker) interactions. We recently developed a new method for studying protein complexes without interfering with the cellular integrity; Virotrap. By trapping and purifying protein complexes in virus-like particles, we avoid cell lysis and preserve intact complexes during the purification process. Here, we present a protein-protein interaction network of the TNFR1-induced NF-κB and apoptosis pathway, using TRADD, FADD, TRAF2, cIAP1, NEMO, TANK, HOIL1, HOIP, SHARPIN, A20, ABIN1 and ABIN2 as baits in Virotrap. Virotrap was applied both under resting conditions as well as upon TNF stimulation, which reveals dynamic interactions in TNFR1-induced NF-κB signaling. The found interactions compromise both known interactors as well as novel ones that might serve as valuable resource for further unraveling the molecular mechanisms controlling inflammation, cellular proliferation and cell death.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:58.986.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009366
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emmy Van Quickelberghe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-29 02:08:14	ID requested
1	2018-11-06 08:30:34	announced
⏵ 2	2024-10-22 04:43:59	announced	2024-10-22: Updated project metadata.

10.6019/PXD009366;

curator keyword: Biological

submitter keyword: Virotrap, NFkB, protein-protein interactions,TNF

Kris Gevaert
contact affiliation	VIB-UGent Center for Medical Biotechnology, Belgium Department of Biochemistry, Ghent University, Belgium
contact email	kris.gevaert@vib-ugent.be
lab head
Emmy Van Quickelberghe
contact affiliation	Ghent University
contact email	emmy.vanquickelberghe@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009366
     1. Label: PRIDE project
     2. Name: protein-protein interaction dataset TNFR1-mediated NFkB signaling