PXD009365-1

PXD009365 is an original dataset announced via ProteomeXchange.

Title	TMT-based Quantitative Proteomics of Developing Sea Buckthorn (Hippophae rhamnoides) Berries Reveals Candidate Proteins Related to Lipid Metabolism
Description	Sea buckthorn (Hippophae rhamnoides L.) is an economically important shrub or small tree indigenous to Asia and Europe. The most well-recognized medicinal and nutraceutical products derive from the berry oil, which is rich in essential fatty acids, monounsaturated palmitoleic acid (16:1 ω-7), linoleic (18:2 ω-6) and alpha-linolenic (18:3 ω-3) acids. In this study, tandem mass tags (TMT) MS/MS were used to investigate protein profiles of lipid metabolism in sea buckthorn berries harvested at 30, 50 and 70 days after flowering. A total of 8626 proteins were detected and 6170 of these were quantified. Further analyses of the proteins and their pathways revealed initiation of fatty acid accumulation throughout the whole berry development. Activity of most of key enzymes, which are related to biosynthesis pathways of triacylglycerol and fatty acids, had peaked at 50 days; but triacylglycerol synthesis through the PDAT (phospholipid: diacylglycerol acyltransferase) catalytic pathway of occurred mostly at the early stage of berry development. Protein expression patterns related to lipid accumulation were also verified at the transcript level using quantitative real-time polymerase chain reaction. The proteome profiles determined here will improve our understanding of the process of lipid accumulation and the dynamic changes in metabolic pathways during sea buckthorn berry development.
HostingRepository	PRIDE
AnnounceDate	2019-04-18
AnnouncementXML	Submission_2019-04-18_06:54:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Green
SpeciesList	scientific name: Hippophae rhamnoides subsp. mongolica; NCBI TaxID: 193528;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-29 02:03:18	ID requested
⏵ 1	2019-04-18 07:07:32	announced

Du W, Xiong CW, Ding J, Nybom H, Ruan CJ, Guo H, Tandem Mass Tag Based Quantitative Proteomics of Developing Sea Buckthorn Berries Reveals Candidate Proteins Related to Lipid Metabolism. J Proteome Res, 18(5):1958-1969(2019) [pubmed]

curator keyword: Biological

submitter keyword: Hippophae rhamnoides, proteomics, lipid metabolism, TMT-based quantitative

Cheng Jiang
contact affiliation	Institute of Plant Resources, Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Nationalities University, Dalian 116600, China
contact email	ruan@dlnu.edu.cn
lab head
Rachel Green
contact affiliation	PTM Biolabs Inc.
contact email	xuan_mao@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009365
     1. Label: PRIDE project
     2. Name: TMT-based Quantitative Proteomics of Developing Sea Buckthorn (Hippophae rhamnoides) Berries Reveals Candidate Proteins Related to Lipid Metabolism