PXD009356-1

PXD009356 is an original dataset announced via ProteomeXchange.

Title	Comparative platelet releasate proteome profiling of acute coronary syndrome versus stable coronary artery disease
Description	Upon activation, platelets release a host of soluble and vesicular signals, collectively termed the ‘platelet releasate’ (PR). The contents of this PR play a significant role in haemostasis, inflammation, and pathologic sequelae. Despite this, proteomic studies investigating the PR in coronary artery disease have not been performed. We undertook a comparative label-free quantitative (LFQ) proteomic profiling of the 1U/ml thrombin-induced PR from 13 acute coronary syndrome (ACS-STEMI) versus 14 stable angina pectoris patients using a tandem mass spectrometry approach. We identified differentially released platet proteins including tetranectin (CLEC3B), protein disulfide-isomerase-A3 (PDIA3), coagulation factor V (F5) and fibronectin (FN1). Strikingly, all 9 differential proteins were associated with the GO cellular component term ‘extracellular vesicle’ and reduced levels of EVs were detected in plasma of ACS-STEMI patients. Network analysis revealed 3 PR proteins either reduced (F5; FN1) or absent (CLEC3B) in ACS-STEMI patients, which are strongly connected to both the clotting cascade and major druggable targets on platelets. This moderated signature highlights the possible basis of platelet dysfunction in ACS-STEMI and may prove useful for non-invasive risk assessment of the progression of coronary artery disease.
HostingRepository	PRIDE
AnnounceDate	2020-06-05
AnnouncementXML	Submission_2020-06-05_03:42:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Szklanna
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-28 05:22:52	ID requested
⏵ 1	2020-06-05 03:42:53	announced
2	2024-10-22 05:06:48	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Acute Coronary Syndrome, Platelets, Platelet Releasate, Proteomics, Stable Coronary Artery Disease, LC-MS/MS

Patricia Maguire
contact affiliation	SBBS, Conway Institute, UCD, Belfield, Dublin
contact email	patricia.maguire@ucd.ie
lab head
Paulina Szklanna
contact affiliation	University College Dublin
contact email	paulina.szklanna@ucdconnect.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD009356

PRIDE project URI

• PRIDE
  1. PXD009356
     1. Label: PRIDE project
     2. Name: Comparative platelet releasate proteome profiling of acute coronary syndrome versus stable coronary artery disease