PXD009295 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | I/L-discrimination by MS3 of dimethylated peptides |
Description | A novel mass spectrometric method for discriminating isoleucine and leucine by MS3. The dimethylation of peptide N-termini leads to intense a1 ions in CID and MS3 fragmentation of the isoleucine/leucine a1-ion leads to spectra with fragments that can discriminate between the two isomers. The method was applied to two antibodies in combination with a set of protease treatments using trypsin, thermolysin, chymotrypsin and pepsin to generate peptides exposing N-terminal I/L residues. |
HostingRepository | PRIDE |
AnnounceDate | 2018-07-24 |
AnnouncementXML | Submission_2018-07-24_09:40:19.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD009295 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jakob Bunkenborg |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Glu->pyro-Glu; Deamidated; Dimethyl; Carbamidomethyl; Oxidation; Dimethyl:2H(4) |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-22 02:41:34 | ID requested | |
⏵ 1 | 2018-07-24 09:40:20 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical |
submitter keyword: LC-MS, isoleucine, leucine, I/L-discrimination, Orbitrap Fusion, MS3, Antibody |
Contact List
Jakob Bunkenborg |
contact affiliation | ALPHALYSE A/S Unsbjergvej 4 • 5220 Odense SØ • Denmark Phone: +45 6310 6505 |
contact email | bunkenborg@alphalyse.com |
lab head | |
Jakob Bunkenborg |
contact affiliation | Alphalyse |
contact email | jakob.bunkenborg@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009295
- Label: PRIDE project
- Name: I/L-discrimination by MS3 of dimethylated peptides