PXD009270-2

PXD009270 is an original dataset announced via ProteomeXchange.

Title	Comprehensive analysis of proteomic landscape remodeling during carcinogenesis
Description	We have performed a comprehensive analysis of how oncogenic transformation induced by v-Src kinase remodels the proteomic landscape of human breast epithelial (MCF10A) cells. We characterised the proteome of untransformed cells to a depth of ~14,000 proteins, spanning a wide dynamic range of expression levels. Changes in both protein abundance and protein turnover were analysed, in biological triplicate, at seven time points spanning 1-72 hours post v-Src activation, coincident with profound phenotypic changes in cell behaviour and morphology. These data are provided for interactive exploration via the Encyclopedia of Proteome Dynamics database (www.peptracker.com/epd). We detect only a small subset (<3%) of mostly low copy number proteins showing altered abundance and/or half-life after transformation, including proteins regulated at the post-transcriptional level. Src activation modulated proteins with diverse cellular functions and with differential response kinetics. The resulting ‘Src signature’ is shown to be prognostic of reduced cancer patient survival post diagnosis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:47.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott Greig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	protonated glycine (glycinium) residue; methylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; monohydroxylated residue; acetylated residue; dimethylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-20 09:34:20	ID requested
1	2018-04-12 08:58:18	announced
⏵ 2	2024-10-22 04:43:48	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Epithelial, Src kinase,MCF10A, Human Breast, half-life

Angus Lamond
contact affiliation	Principal Investigator
contact email	a.i.lamond@dundee.ac.uk
lab head
Scott Greig
contact affiliation	University of Dundee
contact email	sjzgreig@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009270
     1. Label: PRIDE project
     2. Name: Comprehensive analysis of proteomic landscape remodeling during carcinogenesis