PXD009266-1

PXD009266 is an original dataset announced via ProteomeXchange.

Title	A549 overexpressing CCAR1 LC-MS/MS
Description	CCAR1 mRNA have two transcripts, the 3.5 kb full length transcript and the 2.5 kb shorter transcript which contained a deletion in exon 15—exon 22 of full-length CCAR1 cDNA, where sequences mapped to 1196–3120 bp were missed. CCAR1L and CCAR1S shared a common N-terminus and a common C-terminal end, but the latter had a deletion of SAP domain. As a DNA/RNA-binding domain, the SAP motif is involved in chromosomal organization and apoptosis. To explaine the divergence of CCAR1L and CCAR1S in regulating apoptosis,we purified CCAR1L and CCAR1S from flag-CCAR1L or CCAR1S overexpressing A549 cells by performing IP-MS to screening the possible proteins which are specifically interacting with CCAR1L or CCAR1S.
HostingRepository	PRIDE
AnnounceDate	2018-05-15
AnnouncementXML	Submission_2018-05-15_08:15:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huang Qingyang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-20 08:35:47	ID requested
⏵ 1	2018-05-15 08:15:29	announced
2	2018-06-28 23:40:42	announced	Updated publication reference for PubMed record(s): 29942010.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: A549,CCAR1,IP-MS

Zhang Lingqiang
contact affiliation	State Key Laboratory of Proteomics, Beijing Proteome Research Center,
contact email	zhanglq@nic.bmi.ac.cn
lab head
Huang Qingyang
contact affiliation	State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, National Center for Protein Sciences (The PHOENIX Center, Beijing), Beijing 102206, China.
contact email	qingyang89@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD009266

PRIDE project URI

• PRIDE
  1. PXD009266
     1. Label: PRIDE project
     2. Name: A549 overexpressing CCAR1 LC-MS/MS