PXD009258-2

PXD009258 is an original dataset announced via ProteomeXchange.

Title	Absolute proteome quantification of highly purified reticulocytes and erythrocytes
Description	Erythrocytes are the most numerous cells of the human body (Sender 2016). Their lifespan in the blood stream is around 120 days. The turnover implies the production of 2 million new reticulocytes each second. Circulating reticulocytes achieve their maturation in 2 to 3 days. During this short time, important modifications occur such as membrane remodeling, removing of remaining organelles and specific elimination of useless proteins such as the transferrin receptor. This remodeling will ensure the optimization of the erythrocyte ability to fulfil its transport functions. We performed a deep label-free quantitative proteome analysis of purified erythrocytes and reticulocytes to better understand the physiology of the erythrocytes and the last steps of their maturation.
HostingRepository	PRIDE
AnnounceDate	2018-09-18
AnnouncementXML	Submission_2019-06-17_03:26:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilie-Fleur GAUTIER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-19 09:22:43	ID requested
1	2018-09-18 07:13:48	announced
⏵ 2	2019-06-17 03:26:25	announced	Updated publication reference for PubMed record(s): 30327373.
3	2024-10-22 04:44:00	announced	2024-10-22: Updated project metadata.

Gautier EF, Leduc M, Cochet S, Bailly K, Lacombe C, Mohandas N, Guillonneau F, El Nemer W, Mayeux P, Absolute proteome quantification of highly purified populations of circulating reticulocytes and mature erythrocytes. Blood Adv, 2(20):2646-2657(2018) [pubmed]

curator keyword: Biological

submitter keyword: red blood cells, erythrocytes, reticulocytes, Label free quantification, human primary cells

Patrick Mayeux
contact affiliation	Institut Cochin, INSERM U1016, CNRS UMR8104, Plateforme de Proteomique 3P5, GR-Ex, Paris Descartes University
contact email	patrick.mayeux@inserm.fr
lab head
Emilie-Fleur GAUTIER
contact affiliation	Plateforme Proteomique 3P5, Université Paris Descartes, Inserm U1016, Institut Cochin, Cnrs UMR8104, France
contact email	ef.gautier.upd@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009258

PRIDE project URI

• PRIDE
  1. PXD009258
     1. Label: PRIDE project
     2. Name: Absolute proteome quantification of highly purified reticulocytes and erythrocytes