PXD009253-1

PXD009253 is an original dataset announced via ProteomeXchange.

Title	The host proteome reveals nutrient exchange and cell recognition mechanisms altered by a novel cnidarian-Symbiodinium symbiosis.
Description	Symbiosis between corals and their dinoflagellate symbionts supports highly diverse coral reef ecosystems, which are increasingly threatened by anthropogenic climate change. The acquisition of novel, thermotolerant symbionts has been proposed as a natural or assisted mechanism of increasing reef resilience, but the cellular consequences of novel symbiotic associations are unclear. We used liquid chromatography-mass spectrometry to investigate the effects of a novel symbiosis on the host proteome of a model cnidarian. Aposymbiotic Exaiptasia were colonised by either the homologous symbiont (Symbiodinium minutum) or a thermally tolerant, ecologically invasive heterologous symbiont (Symbiodinium trenchii). Cnidarians hosting S. trenchii exhibited minimal expression of Niemann-Pick C2 proteins, which are important in sterol transport and cell recognition, and glutamine synthetases, which are essential for nitrogen recycling. Many proteins utilized by apicomplexans to parasitize mammals were differentially expressed between symbioses and between symbiotic states. The pathways in which these proteins function are likely important determinants of successful symbiosis establishment, and their identification may elucidate the features responsible for the physiologically suboptimal symbiosis with S. trenchii, despite its thermotolerance. The capacity of the two partners to integrate their metabolisms may limit the ability of corals to establish novel, thermally tolerant symbioses in response to climate change.
HostingRepository	PRIDE
AnnounceDate	2019-05-27
AnnouncementXML	Submission_2019-05-27_00:29:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clinton Oakley
SpeciesList	scientific name: Exaiptasia pallida; NCBI TaxID: 1720309;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-19 05:24:57	ID requested
⏵ 1	2019-05-27 00:29:41	announced
2	2019-05-27 02:26:21	announced	Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Symbiodinium, Exaiptasia, coral, symbiosis

Simon K Davy
contact affiliation	School of Biological Sciences Victoria University of Wellington New Zealand
contact email	simon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD009253

PRIDE project URI

• PRIDE
  1. PXD009253
     1. Label: PRIDE project
     2. Name: The host proteome reveals nutrient exchange and cell recognition mechanisms altered by a novel cnidarian-Symbiodinium symbiosis.