PXD009245-1

PXD009245 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	TGFβ-Induced Proteins in Non-Endocrine Mouse Pituitary Cell Line TtT/GF
Description	TtT/GF is a mouse pituitary cell line derived from radiothyroidectomy-induced thyrotropic pituitary tumor and has been recognized as a model of folliculostellate cells. Our previous studies suggested that TtT/GF cells have cellular plasticity, rather than representing a model only for folliculostellate cells. We found that transforming growth factor beta (TGFβ) reinforces pericyte properties (Tsukada et al. Cell and Tissue Research, 371: 339-350, 2018). In order to extensively identify TGFβ-induced proteins in TtT/GF cells, the present study performed mass spectrometry analysis combined with the stable isotope labeling of amino acids in cell culture (SILAC) system. TtT/GF cells were cultured in either light medium (containing normal Arg/Lys) or heavy medium (containing 13C6-labelled Arg/Lys) in the presence of vehicle (light), TGFβ (heavy), or selective TGFβ receptor I inhibitor (SB431542, heavy). Collected proteins were then analyzed by mass spectrometry. We successfully found 51 up-regulated and 112 down-regulated proteins, which are related to actin cytoskeleton, cell adhesion, extracellular matrix, and DNA replication. The result also showed up-regulation of many pericyte markers/pointers, supporting our previous hypothesis. Intriguingly, we found down-regulation of several pituitary adenoma markers. Mass spectrometry data using SILAC will provide valuable information about pathological processes in pituitary adenomas, as well as pericyte differentiation.
HostingRepository	jPOST
AnnounceDate	2019-03-19
AnnouncementXML	Submission_2019-03-18_08:00:05.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yukinobu Isowa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 6x(13)C labeled L-lysine; 6x(13)C labeled L-arginine
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-18 19:23:43	ID requested
⏵ 1	2019-03-18 08:00:05	announced
2	2021-02-04 16:35:10	announced	2021-02-04: Updated FTP location.
3	2022-09-18 02:52:35	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Differentiation, isotopic tracer, pituitary gland, stem cells, transforming growth factor beta (TGFβ), pericyte, folliculostellate cell

submitter keyword: Differentiation, isotopic tracer, pituitary gland, stem cells, transforming growth factor beta (TGFβ), pericyte, folliculostellate cell

Contact List

Takehiro Tsukada
lab head
Yukinobu Isowa
contact affiliation	Meiji university
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000406

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000406