PXD009243-1

PXD009243 is an original dataset announced via ProteomeXchange.

Title	Twenty-four hour rhythms in the absence of the clock gene Bmal1
Description	Circadian (~24 hour) clocks exist in almost all types of living organism and play a fundamental role in regulating daily physiological and behavioural processes. The transcription factor BMAL1 (ARNTL) is thought to be one of the principal drivers of the molecular clock in mammals since its deletion abolishes 24-hour activity patterning, an important physiological output of the clockwork. However, whether or not Bmal1-/- mice can nevertheless display molecular 24-hour rhythms is unknown. Here, we determined whether Bmal1 function is necessary for daily molecular oscillations in two tissues – skin fibroblasts and liver. Unexpectedly, both tissues exhibited robust 24-hour oscillations over 2-3 days in the absence of any exogenous synchronizers such as daily light or temperature cycles. This demonstrates a competent 24-hour molecular pacemaker in Bmal1 knockouts. Indeed, molecular oscillations were pervasive throughout the transcriptome, proteome and phosphoproteome of Bmal1-/- mice. In particular, several proteins exhibited rhythmic phosphorylation in both Bmal1-proficient and -deficient cells, highlighting an unanticipated role for post-translational regulators in 24-hour rhythms in the absence of any known clock mechanisms.
HostingRepository	PRIDE
AnnounceDate	2020-02-17
AnnouncementXML	Submission_2020-02-17_07:21:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandipan Ray
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-16 09:27:02	ID requested
⏵ 1	2020-02-17 07:21:39	announced
2	2024-10-22 04:59:37	announced	2024-10-22: Updated project metadata.

Ray S, Valekunja UK, Stangherlin A, Howell SA, Snijders AP, Damodaran G, Reddy AB, . Science, 367(6479):800-806(2020) [pubmed]

curator keyword: Biological

submitter keyword: Bmal1

Circadian rhythms

Kinase network

Non-transcriptional oscillators

Rhythmic proteome and phosphoproteome

Akhilesh B. Reddy
contact affiliation	The Francis Crick Institute, 1 Midland Road, London NW1 1AT, United Kingdom
contact email	areddy@cantab.net
lab head
Sandipan Ray
contact affiliation	The Francis Crick Institute
contact email	sandipan.ray@cantab.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD009243

PRIDE project URI

• PRIDE
  1. PXD009243
     1. Label: PRIDE project
     2. Name: Twenty-four hour rhythms in the absence of the clock gene Bmal1