PXD009240-2

PXD009240 is an original dataset announced via ProteomeXchange.

Title	Drosophila insulin-like peptides DILP2 and DILP5 stimulation global phosphoproteome
Description	Insulin and IGF signaling (IIS) is a complex system that controls diverse processes including growth, development, metabolism, stress responses and aging. Drosophila melanogaster IIS is propagated by eight Drosophila insulin-like peptides (DILPs), homologues of both mammalian insulin and IGFs, with various spatiotemporal expression patterns and functions. DILPs 1-7 are thought to act through a single Drosophila insulin/IGF receptor, InR, but it is unclear how the DILPs thereby mediate a range of physiological phenotypes. We determined the distinct cell signaling effects of DILP2 and DILP5 stimulation upon Drosophila S2 cells. DILP2 and DILP5 induced similar transcriptional patterns, but differed in signal transduction kinetics. DILP5 induced sustained phosphorylation of Akt, while DILP2 produced acute, transient Akt phosphorylation. Accordingly, we used phosphoproteomic analysis to identify distinct patterns of non-genomic signaling induced by DILP2 and DILP5. Across all treatments and replicates, 5250 unique phosphopeptides were identified, representing 1575 proteins. Among these peptides, DILP2, but not DILP5, dephosphorylated Ser15 on glycogen phosphorylase (GlyP), and DILP2, but not DILP5, was subsequently shown to repress enzymatic GlyP activity in S2 cells. The functional consequences of this difference were evaluated in adult Drosophila dilp mutants: dilp2 null adults have elevated GlyP enzymatic activity relative to wildtype, while dilp5 mutants have reduced GlyP activity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:44:06.344.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephanie Post
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: NCBITaxon:7227; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-16 07:54:44	ID requested
1	2018-10-19 11:42:13	announced
⏵ 2	2024-10-22 04:44:11	announced	2024-10-22: Updated project metadata.

Post S, Karashchuk G, Wade JD, Sajid W, De Meyts P, Tatar M, Insulin-Like Peptides DILP2 and DILP5 Differentially Stimulate Cell Signaling and Glycogen Phosphorylase to Regulate Longevity. Front Endocrinol (Lausanne), 9():245(2018) [pubmed]

10.3389/fendo.2018.00245;

curator keyword: Biological

submitter keyword: insulin-like peptide, insulin/IGF signaling, DILP, Drosophila

Marc Tatar
contact affiliation	Department of Ecology and Evolutionary Biology, Brown University, United States
contact email	marc_tatar@brown.edu
lab head
Stephanie Post
contact affiliation	Brown University
contact email	stephanie_post@brown.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009240
     1. Label: PRIDE project
     2. Name: Drosophila insulin-like peptides DILP2 and DILP5 stimulation global phosphoproteome