PXD009235-1

PXD009235 is an original dataset announced via ProteomeXchange.

Title	Clostridium sordellii outer spore proteins
Description	Bacterial spores play an important role in disease initiation, transmission and persistence. The outermost spore layer, the exosporium, is important as it is the first point of contact between the spore and the environment and may be involved in spore adherence, protection and germination. Clostridium sordellii is a highly lethal, spore forming pathogen that causes soft-tissue infections, enteritis and toxic-shock syndrome. Despite the importance of C. sordellii spores in disease, spore proteins from this bacterium have not been defined or interrogated functionally. In this study, we identified the C. sordellii outer spore proteome and two of the identified proteins, CSA and CSB, were characterised using a genetic and phenotypic approach. Both proteins were essential for the correct formation and positioning of the C. sordellii spore coat and exosporium. The absence of CSA reduced sporulation levels and increased spore sensitivity to heat, sodium hydroxide and hydrochloric acid. By comparison, CSB was required for normal levels of spore adherence to cervical, but not vaginal, cells, with csb mutant spores having increased adherence properties. The establishment of a mouse infection model of the gastrointestinal tract for C. sordellii allowed the role of CSA and CSB to be interrogated in an infected host. Following the oral administration of spores to mice, the wild-type strain efficiently colonized the gastrointestinal tract, with the peak of bacterial numbers occurring at one day post-infection. Colonization was reduced by two logs at four days post-infection. By comparison, mice infected with the csb mutant did not show a reduction in bacterial numbers. The absence of CSB therefore allows the csb mutant to persist within the gastrointestinal tract. We conclude that C. sordellii outer spore proteins are important for the structural and functional integrity of spores, and for colonization and persistence during infection. Finally, Clostridium difficile, Bacillus cereus and Bacillus anthracis encode proteins with homology to CSA and CSB but these bacteria produce spores that are structurally dissimilar to those of C. sordellii, and the function of the proteins in these hosts is different to that in C. sordellii. These findings suggest that, despite their homology, spore proteins can have variable functions in different bacterial species which highlights the necessity of studying each spore protein in the cognate species from which it originates.
HostingRepository	PRIDE
AnnounceDate	2018-05-01
AnnouncementXML	Submission_2018-05-01_06:06:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rommel Mathias
SpeciesList	scientific name: Paeniclostridium sordellii ATCC 9714; NCBI TaxID: 1292036;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-16 07:30:44	ID requested
⏵ 1	2018-05-01 06:06:27	announced
2	2024-10-22 04:43:57	announced	2024-10-22: Updated project metadata.

Rabi R, Larcombe S, Mathias R, McGowan S, Awad M, Lyras D, Clostridium sordellii outer spore proteins maintain spore structural integrity and promote bacterial clearance from the gastrointestinal tract. PLoS Pathog, 14(4):e1007004(2018) [pubmed]

curator keyword: Biological

submitter keyword: Clostridium sordellii, spore, bacteria

Rommel Mathias
contact affiliation	Monash University
contact email	rommel.mathias@monash.edu
lab head
Rommel Mathias
contact affiliation	Monash University
contact email	rommel.mathias@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009235
     1. Label: PRIDE project
     2. Name: Clostridium sordellii outer spore proteins