PXD009225 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteome of Francicella Tularensis |
Description | The pathogenic bacterium Francisella tularensis possesses a non-canonical T6SS encoded on the Francisella pathogenicity island (FPI) that is essential for efficient phagosomal escape and access to the cytosolic compartment of infected host cells. Using a global and site-specific phosphoproteomic analysis of F. novicida, we identified a phosphorylated form of IglB, which constitutes with iglA the outer sheath of the T6SS. We show here that substitution of the unique phosphorylated tyrosine (Y139) of IglB to alanine or to the non-phosphorylatable analogue phenylalanine prevents the formation of the sheath-like structures and impairs normal bacterial phagosomal escape. We propose that IglB phosphorylation is involved in the dynamics of assembly-disassembly of the sheath. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-29 |
AnnouncementXML | Submission_2021-03-29_09:49:35.982.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chiara guerrera |
SpeciesList | scientific name: Francisella tularensis subsp. novicida (strain U112); NCBI TaxID: 401614; |
ModificationList | phosphorylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-15 09:31:58 | ID requested | |
⏵ 1 | 2021-03-29 09:49:36 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Francicella Tularensis, phospho |
Contact List
GUerrera I C |
contact affiliation | Proteomics Laboratory Structure Federative Recherche Necker, INserm US24Paris , France |
contact email | chiara.guerrera@inserm.fr |
lab head | |
Chiara guerrera |
contact affiliation | Necker proteomics, INSERM |
contact email | chiara.guerrera@inserm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009225
- Label: PRIDE project
- Name: Phosphoproteome of Francicella Tularensis