PXD009190-2

PXD009190 is an original dataset announced via ProteomeXchange.

Title	A 17 kDa fragment of lactoferrin associates with the termination of inflammation and peptides within promote resolution
Description	During the resolution of inflammation macrophages engulf apoptotic polymorphonuclear cells (PMN) and can accumulate large numbers of their corpses. Here we report that resolution-phase-macrophages acquire the neutrophil-derived glycoprotein lactoferrin (Lf) in vivo and ex vivo and process it to short fragments. During the onset and resolving phases of inflammation in murine peritonitis and bovine mastitis Lf fragments of 15 and 17 kDa occurred in various body fluids, and the murine fragmentation and release were mediated by macrophages. The 17 kDa fragment contained two bioactive tripeptides, FKD and FKE that promoted human macrophage reprogramming to a pro-resolving phenotype. At low concentrations (1-10 M) this was reflected by inhibition of LPS-induced TNFand IL-6 secretion and increased IL-10 levels. Both peptides inhibited ERK and cJun activation following macrophage exposure to LPS. In addition, FKD, and to a lesser extent FKE, promoted neutrophil-mediated resolution at high concentrations (30-100 M) by enhancing the formation of cytokine-scavenging aggregated NETs at low cellular density. Thus, PMN lactoferrin is acquired, processed and
HostingRepository	PRIDE
AnnounceDate	2018-03-20
AnnouncementXML	Submission_2019-02-12_08:39:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sagie Schif
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-12 12:41:50	ID requested
1	2018-03-20 01:59:01	announced
⏵ 2	2019-02-12 08:39:20	announced	Updated publication reference for PubMed record(s): 29643857.
3	2024-10-22 04:43:41	announced	2024-10-22: Updated project metadata.

Lutaty A, Soboh S, Schif-Zuck S, Zeituni-Timor O, Rostoker R, Podolska MJ, Schauer C, Herrmann M, Mu, ñ, oz LE, Ariel A, A 17-kDa Fragment of Lactoferrin Associates With the Termination of Inflammation and Peptides Within Promote Resolution. Front Immunol, 9():644(2018) [pubmed]

curator keyword: Biological

submitter keyword: Resolution of Inflammation, lactoferrin, macrophages, efferocytosis, NETosis

Amiram Ariel
contact affiliation	The Laboratory for Molecular Pathways in the Resolution of Inflammation, The Departments of Biology and Human Biology, University of Haifa, Israel
contact email	Amiram@research.haifa.ac.il
lab head
Sagie Schif
contact affiliation	Haifa University
contact email	z.sagie@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009190
     1. Label: PRIDE project
     2. Name: A 17 kDa fragment of lactoferrin associates with the termination of inflammation and peptides within promote resolution