PXD009186-2

PXD009186 is an original dataset announced via ProteomeXchange.

Title	Nicotiana tabacum seed lipid droplet proteome
Description	The number of known proteins associated with plant lipid droplets (LDs) is small compared to other organelles. Many questions of LD biosynthesis and degradation remain open, also due to lack of candidate LD proteins whose characterization could help to elucidate their function in those processes. We performed a proteomic screen on LDs isolated from Nicotiana tabacum pollen tubes. Proteins that were highly enriched in the LD fraction compared to the total or cytosolic fraction where verified for LD localization via transient expression in tobacco pollen tubes. We also compared the isoforms of typical LD proteins found in the pollen tubes on a qualitative level to the isoforms found in tobacco seeds.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:58.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-12 07:08:31	ID requested
1	2018-08-13 09:26:38	announced
⏵ 2	2024-10-22 04:33:59	announced	2024-10-22: Updated project metadata.

10.1105/tpc.18.00276;

Kretzschmar FK, Mengel LA, M, ü, ller AO, Schmitt K, Blersch KF, Valerius O, Braus GH, Ischebeck T, PUX10 Is a Lipid Droplet-Localized Scaffold Protein That Interacts with CELL DIVISION CYCLE48 and Is Involved in the Degradation of Lipid Droplet Proteins. Plant Cell, 30(9):2137-2160(2018) [pubmed]

curator keyword: Biological

submitter keyword: tobacco,lipid droplets, seeds

Dr. Till Ischebeck
contact affiliation	Department of Plant Biochemistry Georg-August-Universität Göttingen Justus-von-Liebig Weg 11 37077 Göttingen Germany Tel. +49-551-39-14430
contact email	tischeb@gwdg.de
lab head
Till Ischebeck
contact affiliation	University of Münster
contact email	tischeb@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009186

PRIDE project URI

• PRIDE
  1. PXD009186
     1. Label: PRIDE project
     2. Name: Nicotiana tabacum seed lipid droplet proteome