PXD009130-2
PXD009130 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Universal plant phosphoproteomics workflow and its application to tomato signaling in response to cold stress |
| Description | Phosphorylation-mediated signaling transduction plays a crucial role in the regulation of plant defense mechanisms against environmental stresses. In order to address the high complexity and dynamic range of plant proteomes and phosphoproteomes, we present a universal sample preparation procedure to facilitate profiling plant phosphoproteomes. This advanced workflow significantly improves phosphopeptide identifications, enabling deep insight into plant phosphoproteomes. We then applied the workflow to study the phosphorylation events involved in tomato cold tolerance mechanisms. Phosphoproteomic changes of two tomato species (N135 Green Gage and Atacames) with distinct cold tolerance phenotypes were profiled under cold stress. In total, we identified more than 30,000 unique phosphopeptides from tomato leaves, representing about 5,500 phosphoproteins, as the largest tomato phosphoproteomic resource. The data, along with the validation through in vitro kinase reactions, allowed us to identify kinases involved in cold tolerant signaling and discover distinctive kinase-substrate events in two tomato species under cold environment. In particular, the activation of SnRK2s and their direct substrates may assist N135 Green Gage tomatoes in surviving long-term cold stress. Taken together, the streamlined approach and the resulting deep phosphoproteomic analyses revealed a global view of tomato cold-induced signaling mechanisms. |
| HostingRepository | jPOST |
| AnnounceDate | 2019-03-08 |
| AnnouncementXML | Submission_2021-02-04_16:38:26.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chuan-Chih Hsu |
| SpeciesList | scientific name: Solanum lycopersicum; NCBI TaxID: 4081; scientific name: Solanum pimpinellifolium; NCBI TaxID: 4084; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; alpha-amino acetylated residue; unknown modification; unknown modification; unknown modification |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-03-07 10:07:33 | ID requested | |
| 1 | 2019-03-07 07:00:06 | announced | |
| ⏵ 2 | 2021-02-04 16:38:27 | announced | 2021-02-04: Updated FTP location. |
| 3 | 2022-09-18 03:55:22 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Phosphorylation |
| tomato phosphoproteome |
| mass spectrometry |
| cold stress |
| signal transduction |
Contact List
| Dr. W. Andy Tao | |
|---|---|
| lab head | |
| Chuan-Chih Hsu | |
| contact affiliation | Purdue University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000399 |
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