PXD009095-2

PXD009095 is an original dataset announced via ProteomeXchange.

Title	Molecular basis of Tousled-Like-Kinase 2 (TLK2) activation – LC-MS/MS-based analysis of HEK293-expressed TLK2 phosphorylation state
Description	In this study on human Tousled-Like-Kinase 2 (TLK2) mass spectrometry-based analysis was applied as part of a molecular characterization of TLK2 in the aim to increase the understanding of the mode of activation of this protein. Specifically, different TLK2 constructs were expressed analyzed to generate a “phosphorylation map” of the TLK2 protein. To analyse the phosphorylation state of TLK2 we used recombinant protein purified from HEK293. Samples of different TLK2 constructs were used for in-gel digestion and analysed by mass spectrometry. All samples were prepared and run in triplicates for label-free quantification. Explanation for MS raw files with TLK2 construct included: KD: Kinase-dead TLK2 construct with mutation D613A, N-terminal His- and Strep-tag. wt: Active form of TLK2, canonical sequence, N-terminal His- and Strep-tag. full: Full-length canonical sequence (1-772), N-terminal His- and Strep-tag. trunc: Truncated TLK2 sequence (191-772), N-terminal His- and Strep-tag.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:41.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrick Ruether
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-02 03:07:15	ID requested
1	2018-09-04 08:11:03	announced
⏵ 2	2024-10-22 04:45:48	announced	2024-10-22: Updated project metadata.

Mortuza GB, Hermida D, Pedersen AK, Segura-Bayona S, L, ó, pez-M, é, ndez B, Redondo P, R, ü, ther P, Pozdnyakova I, Garrote AM, Mu, ñ, oz IG, Villamor-Pay, à M, Jauset C, Olsen JV, Stracker TH, Montoya G, Molecular basis of Tousled-Like Kinase 2 activation. Nat Commun, 9(1):2535(2018) [pubmed]

10.1038/s41467-018-04941-y;

curator keyword: Biological

submitter keyword: Human, TLK2, HEK293, Phosphosites

Jesper Velgaard Olsen
contact affiliation	NNF Center for Protein Research, University of Copenhagen
contact email	jesper.olsen@cpr.ku.dk
lab head
Patrick Ruether
contact affiliation	NNF Center for Protein Research - University of Copenhagen
contact email	patrick.ruether@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009095

PRIDE project URI

• PRIDE
  1. PXD009095
     1. Label: PRIDE project
     2. Name: Molecular basis of Tousled-Like-Kinase 2 (TLK2) activation – LC-MS/MS-based analysis of HEK293-expressed TLK2 phosphorylation state