PXD008985-1

PXD008985 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation
Description	Cellular heterogeneity is important to biological processes, including cancer and development. However, proteome heterogeneity is largely unexplored because of the limitations of existing methods for quantifying protein levels in single cells. To alleviate these limitations, we developed Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS), and validated its ability to identify distinct human cancer cell types based on their proteomes. We used SCoPE-MS to quantify thousands of proteins in differentiating mouse embryonic stem (ES) cells. The single-cell proteomes enabled us to deconstruct cell populations and infer protein abundance relationships. Comparison between single-cell proteomes and transcriptomes indicated coordinated mRNA and protein covariation. Yet many genes exhibited functionally concerted and distinct regulatory patterns at the mRNA and the protein levels, suggesting that post-transcriptional regulatory mechanisms contribute to proteome remodeling during lineage specification, especially for developmental genes. SCoPE-MS is broadly applicable to measuring proteome configurations of single cells and linking them to functional phenotypes, such as cell type and differentiation potentials.
HostingRepository	MassIVE
AnnounceDate	2018-02-19
AnnouncementXML	Submission_2018-02-19_08:55:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikolai Slavov
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Label:13C(4)+Oxidation
Instrument	LTQ Orbitrap Elite; Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-19 08:34:58	ID requested
⏵ 1	2018-02-19 08:55:52	announced

Publication List

Budnik B., Levy E., Harmange G., Slavov N. Mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. https://doi.org/10.1101/102681.

Budnik B., Levy E., Harmange G., Slavov N. Mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. https://doi.org/10.1101/102681.

Keyword List

submitter keyword: stem cells, cancer, single cell, low input, TMT

submitter keyword: stem cells, cancer, single cell, low input, TMT

Contact List

Nikolai Slavov
contact affiliation	Northeastern University
contact email	n.slavov@northeastern.edu
lab head
Nikolai Slavov
contact affiliation	Harvard University
contact email	nslavov@alum.mit.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082077

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082077