PXD008906-1

PXD008906 is an original dataset announced via ProteomeXchange.

Title	Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury
Description	Scar formation after brain injury is still ill understood. Here we examined the interplay between astrocyte proliferation and monocyte invasion. Using genetic mouse models to decrease or increase reactive astrocyte proliferation, we demonstrate inverse effects on monocyte numbers in the injury site, with e.g. reduced monocyte numbers when astrocyte proliferation was increased. Conversely, reducing monocyte invasion using CCR2-/- mice causes a strong increase in astrocyte proliferation, demonstrating an intriguing negative cross-regulation between these cell types at the vascular interface. CCR2-/- mice showed reduced scar formation with less extracellular matrix deposition, smaller lesion site and increased neuronal coverage. Surprisingly, also the GFAP+ scar area was significantly decreased despite increased astrocyte proliferation. Proteomic analysis at the peak of increased astrocyte proliferation revealed a decrease in ECM synthesizing enzymes in the injury sites of CCR2-/- mice already at this stage, highlighting how early key aspects of scar formation are initiated. Taken together we provide key novel insights into the cross-regulation of juxtavascular proliferating astrocytes and invading monocytes as a key mechanism of scar formation upon brain injury.
HostingRepository	PRIDE
AnnounceDate	2018-04-16
AnnouncementXML	Submission_2018-04-16_02:04:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliane Merl-Pham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-09 03:08:50	ID requested
⏵ 1	2018-04-16 02:04:52	announced
2	2024-10-22 04:42:58	announced	2024-10-22: Updated project metadata.

Frik J, Merl-Pham J, Plesnila N, Mattugini N, Kjell J, Kraska J, G, ó, mez RM, Hauck SM, Sirko S, G, ö, tz M, Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury. EMBO Rep, 19(5):(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Brain, LC-MSMS

Magdalena Götz
contact affiliation	Institute for Stem Cell Research, Helmholtz Center Munich, Germany
contact email	magdalena.goetz@helmholtz-muenchen.de
lab head
Juliane Merl-Pham
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008906

PRIDE project URI

• PRIDE
  1. PXD008906
     1. Label: PRIDE project
     2. Name: Cross-talk between monocyte invasion and astrocyte proliferation regulates scarring in brain injury