PXD008905-1

PXD008905 is an original dataset announced via ProteomeXchange.

Title	Characterization of ESX-1 components EccA1, EspG1 and EspH reveal pivotal role of Esp substrates in the Mycobacterium marinum infection cycle
Description	The pathogen Mycobacterium tuberculosis employs a range of ESX-1 substrates to manipulate the host and build a successful infection. Although the importance of ESX-1 secretion in virulence is well established, the characterization of its individual components and the role of individual substrates is far from complete. Here, we describe the functional characterization of the accessory ESX-1 proteins EccA1, EspG1 and EspH, i.e. proteins that are not present in all five ESX system of mycobacteria. Proteomic analysis revealed that EspG1 is crucial for ESX-1 secretion, since all detectable ESX-1 substrates were absent from the cell surface and culture supernatant in an espG1 mutant. Deletion of eccA1 resulted in minor secretion defects, but interestingly, the severity of these secretion defects was dependent on the culture conditions. Finally, espH deletion showed a partial secretion defect, only secretion of EspE, EspF and EsxA/EsxB was blocked.
HostingRepository	PRIDE
AnnounceDate	2018-08-02
AnnouncementXML	Submission_2018-08-02_03:43:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sander Piersma
SpeciesList	scientific name: Mycobacterium marinum ATCC BAA-535; NCBI TaxID: 1348799;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-08 08:10:59	ID requested
⏵ 1	2018-08-02 03:43:17	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Mycobactrium marinum, ESX-1, secretion, EccA1

EspG1

EspH

EsxA, label-free quantitation

Connie Ramona Jimenez
contact affiliation	OncoProteomics Laboratory, Dept of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
contact email	c.jimenez@vumc.nl
lab head
Sander Piersma
contact affiliation	OncoProteomics Laboratory, dept of Medical Oncology, VUmc Medical Center, Amsterdam, The Netherlands
contact email	s.piersma@vumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD008905

PRIDE project URI

• PRIDE
  1. PXD008905
     1. Label: PRIDE project
     2. Name: Characterization of ESX-1 components EccA1, EspG1 and EspH reveal pivotal role of Esp substrates in the Mycobacterium marinum infection cycle