PXD008904-1

PXD008904 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of susceptible and resistance maize lines to southern corn rust
Description	Southern corn rust (SCR) is one of destructive diseases in maize caused by Puccinia polysora Undrew. (P. polysara), widely occurring in warm-temperate and tropical regions globally. To identify candidate SCR resistance-related proteins and understand the molecular mechanism underlaying the maize and P. polysara interaction, comparative proteomic analysis of susceptible and resistance maize lines was performed. A total of 6,612 proteins were successfully identified using an iTRAQ-based proteomic approach. Fold changes and statistical analysis demonstrated that 687 proteins increased and 802 proteins decreased in the resistant line, while 571 increased and 464 decreased in the susceptible line. One remorin protein, namely ZmREM1.3 (B4G1B0), was significantly induced by SCR in the resistant genotype, while decreased in susceptible genotype after P. polysara infection. Plant-specific remorin proteins have been shown to play important roles during microbial infection and plant signaling processes. Transgenic analysis showed that overexpression of ZmREM1.3 in maize confers enhanced resistance to the biotrophic fungal pathogen SCR. Upon pathogen challenge, the ZmREM1.3-overexpressing plants accumulated higher levels of defense hormones, SA and JA. Moreover, stronger induction of defense gene expression was also observed in ZmREM1.3-overexpressing maize plants in response to SCR infection. Taken together, our results support that ZmREM1.3 plays a positive role in regulating the maize defense against SCR likely through SA/JA-mediated defense signaling pathways. This is the first attempt for large scale analysis of the molecular mechanisms underlaying the maize and P. polysara interaction at the proteomic level, and the first evidence for remorin protein family in resistant to fungal disease.
HostingRepository	PRIDE
AnnounceDate	2019-04-18
AnnouncementXML	Submission_2019-04-18_02:00:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shunxi Wang
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-08 07:55:37	ID requested
⏵ 1	2019-04-18 02:00:45	announced
2	2024-10-22 04:43:02	announced	2024-10-22: Updated project metadata.

Wang S, Chen Z, Tian L, Ding Y, Zhang J, Zhou J, Liu P, Chen Y, Wu L, Comparative proteomics combined with analyses of transgenic plants reveal ZmREM1.3 mediates maize resistance to southern corn rust. Plant Biotechnol J, 17(11):2153-2168(2019) [pubmed]

curator keyword: Biological

submitter keyword: Southern corn rust, maize, proteomic

Liuji Wu
contact affiliation	Henan agricultural university, China
contact email	wlj200120@163.com
lab head
Shunxi Wang
contact affiliation	Henan agricultural university
contact email	wangshunxi2008@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008904
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of susceptible and resistance maize lines to southern corn rust