PXD008881-2

PXD008881 is an original dataset announced via ProteomeXchange.

Title	Bottom-up and top-down LC-MS/MS of ribosomal purifications
Description	Ribosomal purifications of spinach 70S ribosome and human 40S and 60S ribosomal subunits were analyzed with bottom-up LC-MS/MS to identify proteins comprising purified complexes as well as co-purified proteins. In order to assess and quantify proteoforms of ribosomal proteins in ribosomal purifications we employed top-down LC-MS/MS techniques with optimized methods, wherein apart from standard parameters (e.g. collision voltage and dynamic exclusion time) high-resolution and low-resolution were alternately employed in full MS mode.
HostingRepository	PRIDE
AnnounceDate	2018-06-26
AnnouncementXML	Submission_2018-07-02_07:18:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sem Tamara
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Spinacia oleracea; NCBI TaxID: 3562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-07 01:41:20	ID requested
1	2018-06-26 06:37:05	announced
⏵ 2	2018-07-02 07:18:40	announced	Updated publication reference for PubMed record(s): 29950687.

van de Waterbeemd M, Tamara S, Fort KL, Damoc E, Franc V, Bieri P, Itten M, Makarov A, Ban N, Heck AJR, Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods. Nat Commun, 9(1):2493(2018) [pubmed]

curator keyword: Biological

submitter keyword: ribosome, top-down, bottom-up

Albert J. R. Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands
contact email	a.j.r.heck@uu.nl
lab head
Sem Tamara
contact affiliation	Utrecht University
contact email	s.tamara@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008881

PRIDE project URI

• PRIDE
  1. PXD008881
     1. Label: PRIDE project
     2. Name: Bottom-up and top-down LC-MS/MS of ribosomal purifications