PXD008870-1

PXD008870 is an original dataset announced via ProteomeXchange.

Title	Intestinal metaproteomics reveals host-microbiota interactions in subjects at risk for type 1 diabetes
Description	Dysbiosis of the gut microbiota has been linked to disease pathogenesis in type 1 diabetes (T1D), yet the functional consequences to the host of this dysbiosis is unknown. Here, we have performed a metaproteomic analysis of 103 stool samples from subjects that either had recent-onset T1D, were high-risk autoantibody positive or low-risk autoantibody negative relatives of individuals with beta cell autoimmunity or healthy individuals to identify signatures in host and microbial proteins associated with disease risk. Multivariate modelling analysis demonstrated that both human host proteins and microbial derived proteins could be used to differentiate new-onset and seropositive individuals from low-risk and healthy controls. Significant alterations were identified between subjects with T1D or islet autoimmunity versus autoantibody negative and control subjects in the prevalence of individual host proteins associated with exocrine pancreas function, inflammation and mucosal function. Data integrationIntegrative analysis combining the metaproteomic data with bacterial abundance showed that taxa that were depleted in new-onset T1D patients were positively associated with host proteins involved in maintaining function of the mucous barrier, microvilli adhesion and exocrine pancreas. These data support the notion that T1D patients have increased intestinal inflammation and decreased barrier function. They also confirmed that pancreatic exocrine dysfunction occurs in new-onset T1D patients and show for the first time that this dysfunction is present in high-risk individuals prior to disease onset. Our data has identified a unique T1D-associated signature in stool that may be useful as a means to monitor disease progression or response to therapies aimed at restoring a healthy microbiota.
HostingRepository	PRIDE
AnnounceDate	2018-11-07
AnnouncementXML	Submission_2018-11-07_01:21:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	patrick gavin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-06 08:05:39	ID requested
⏵ 1	2018-11-07 01:21:09	announced

Gavin PG, Mullaney JA, Loo D, Cao KL, Gottlieb PA, Hill MM, Zipris D, Hamilton-Williams EE, Intestinal Metaproteomics Reveals Host-Microbiota Interactions in Subjects at Risk for Type 1 Diabetes. Diabetes Care, 41(10):2178-2186(2018) [pubmed]

curator keyword: Metaproteomics

submitter keyword: diabetes metaproteome

Emma Hamilton-Williams
contact affiliation	The University of Queensland Diamantina Institute, Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia
contact email	e.hamiltonwilliams@uq.edu.au
lab head
patrick gavin
contact affiliation	university of queensland
contact email	p.gavin@uq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008870
     1. Label: PRIDE project
     2. Name: Intestinal metaproteomics reveals host-microbiota interactions in subjects at risk for type 1 diabetes