PXD008857-2

PXD008857 is an original dataset announced via ProteomeXchange.

Title	PSI supercomplex copurification using PSI His-tag
Description	Chlamydomonas reinhardtii PSI-Cyt b6f complexes from sucrose density gradient fractions were identified by western blot and SMF spectroscopy, but showed high heterogeneity, as evident from the multiple bands present in the Coomassie stained SDS gel and original EM micrographs. Therefore potential PSI-Cyt b6f supercomplexes were enriched by a subsequent PsaA HisTag purification step. Only this additional enrichment enabled the identification of novel PSI supercomplexes, although the sample remained far from being homogeneous. This dataset describes the quantitative mass spectrometric analysis of the enriched PSI supercomplex fraction in comparison to unpurified samples using metabolic 15N labeling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:59:31.503.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-05 07:27:20	ID requested
1	2018-10-03 08:48:08	announced
⏵ 2	2024-10-22 03:59:34	announced	2024-10-22: Updated project metadata.

10.1073/pnas.1809973115;

Steinbeck J, Ross IL, Rothnagel R, G, ä, belein P, Schulze S, Giles N, Ali R, Drysdale R, Sierecki E, Gambin Y, Stahlberg H, Takahashi Y, Hippler M, Hankamer B, promoting cyclic electron flow under anaerobic conditions. Proc Natl Acad Sci U S A, 115(41):10517-10522(2018) [pubmed]

curator keyword: Biological

submitter keyword: His-tag,PSI, supercomplex, Chlamydomonas reinhardtii

Michael Hippler
contact affiliation	Institute of Plant Biology and Biotechnology, University of Muenster
contact email	mhippler@uni-muenster.de
lab head
Stefan Schulze
contact affiliation	University of Pennsylvania
contact email	sschulze@sas.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008857

PRIDE project URI

• PRIDE
  1. PXD008857
     1. Label: PRIDE project
     2. Name: PSI supercomplex copurification using PSI His-tag