PXD008842-1

PXD008842 is an original dataset announced via ProteomeXchange.

Title	Endoplasmic reticulum di-glycine modified proteome under p97/VCP inhibition
Description	Endoplasmic reticulum (ER)-associated degradation (ERAD) mediates the proteasomal clearance of proteins from the early secretory pathway. In this process, ubiquitinated substrates are extracted from membrane-embedded dislocation complexes by the AAA ATPase VCP and targeted to the cytosolic 26S proteasome. In addition to its well-established role in the degradation of misfolded proteins, ERAD also regulates the abundance of key proteins such as enzymes involved in cholesterol synthesis. However, due to the lack of generalizable methods, our understanding of the scope of proteins regulated targeted by ERAD remains limited. To overcome this obstacle, we developed a VCP-inhibitor substrate trapping approach (VISTA) to identify endogenous ERAD substrates. VISTA exploits the small-molecule VCP inhibitor CB5083 to trap ERAD substrates in a membrane-associated, ubiquitinated form.
HostingRepository	PRIDE
AnnounceDate	2018-03-12
AnnouncementXML	Submission_2018-03-12_02:04:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Olzmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	6410 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-02 04:23:48	ID requested
⏵ 1	2018-03-12 02:04:25	announced

Huang EY, To M, Tran E, Dionisio LTA, Cho HJ, Baney KLM, Pataki CI, Olzmann JA, A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates. Mol Biol Cell, 29(9):1021-1030(2018) [pubmed]

curator keyword: Biological

submitter keyword: ERAD, digly, ubiquitin

James Olzmann
contact affiliation	Department of Nutritional Sciences and Toxicology University of California, Berkeley Berkeley, CA
contact email	jolzmann@gmail.com
lab head
James Olzmann
contact affiliation	University of California, Berkeley
contact email	jolzmann@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008842
     1. Label: PRIDE project
     2. Name: Endoplasmic reticulum di-glycine modified proteome under p97/VCP inhibition